| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MIER2 |
| Uniprot No | Q8N344 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-545aa |
| 氨基酸序列 | MAEASSLGRQSPRVVSCLEHSLCPGEPGLQTTAVVSMGSGDHQFNLAEILSQNYSVRGECEEASRCPDKPKEELEKDFISQSNDMPFDELLALYGYEASDPISDRESEGGDVAPNLPDMTLDKEQIAKDLLSGEEEEETQSSADDLTPSVTSHEASDLFPNRSGSRFLADEDREPGSSASSDTEEDSLPANKCKKEIMVGPQFQADLSNLHLNRHCEKIYENEDQLLWDPSVLPEREVEEFLYRAVKRRWHEMAGPQLPEGEAVKDSEQALYELVKCNFNVEEALRRLRFNVKVIRDGLCAWSEEECRNFEHGFRVHGKNFHLIQANKVRTRSVGECVEYYYLWKKSERYDYFAQQTRLGRRKYVPSGTTDADQDLDGSDPDGPGRPRPEQDTLTGMRTDPLSVDGTAGGLDEPGVASDGLPSSEPGPCSFQQLDESPAVPLSHRPPALADPASYQPAVTAPEPDASPRLAVDFALPKELPLISSHVDLSGDPEETVAPAQVALSVTEFGLIGIGDVNPFLAAHPTCPAPGLHSEPLSHCNVMTC |
| 预测分子量 | 65.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于MIER2重组蛋白的研究文献目前较为有限，以下为模拟示例(非真实文献)，供参考格式：
1. **文献名称**：*Characterization of MIER2 Recombinant Protein in Transcriptional Regulation*
**作者**：Smith A, et al.
**摘要**：研究通过大肠杆菌系统表达并纯化MIER2重组蛋白，发现其通过结合特定DNA序列抑制Wnt信号通路，可能参与胚胎发育调控。
2. **文献名称**：*MIER2 Interaction with Histone Deacetylases: A Functional Study*
**作者**：Lee B, et al.
**摘要**：利用重组MIER2蛋白进行体外互作实验，证实其与HDAC1/2形成复合物，调节染色质结构并影响细胞分化过程。
3. **文献名称**：*Development of a MIER2 Knockout Model Using Recombinant Protein-Based Screening*
**作者**：Chen C, et al.
**摘要**：通过重组MIER2蛋白制备抗体，构建基因敲除小鼠模型，发现MIER2缺失导致神经管发育异常，提示其在神经系统中的作用。
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**说明**：以上为模拟内容，实际文献需通过PubMed、Web of Science等平台检索关键词“MIER2 recombinant protein”或“MIER2 protein function”获取。建议结合具体研究领域(如癌症、发育生物学)缩小范围。
**Background of MIER2 Recombinant Protein**
MIER2 (Mesoderm Induction Early Response 2) is a member of the MIER family of transcriptional regulators, which play critical roles in modulating chromatin structure and gene expression. Initially identified for its involvement in early mesoderm induction, MIER2 has since been linked to diverse cellular processes, including cell proliferation, differentiation, and apoptosis. Structurally, MIER2 contains conserved domains such as an N-terminal acidic region, a central SANT domain (implicated in chromatin remodeling and protein interactions), and a C-terminal phosphorylation site. These features enable MIER2 to act as a transcriptional co-regulator, interacting with histone deacetylases (HDACs), histone acetyltransferases, and chromatin-modifying complexes like Sin3A/HDAC to fine-tune gene silencing or activation.
Recombinant MIER2 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional MIER2 for experimental studies. It often includes tags (e.g., His or GST tags) to facilitate purification and detection. Research utilizing recombinant MIER2 has shed light on its dual roles in cancer biology, where it may act as a tumor suppressor or oncogenic driver depending on cellular context. For instance, MIER2 dysregulation has been associated with breast, colorectal, and liver cancers, influencing pathways like Wnt/β-catenin and TGF-β signaling.
Current studies focus on elucidating MIER2’s molecular mechanisms, including its post-translational modifications (e.g., phosphorylation) and interactions with DNA repair or epigenetic machinery. Recombinant MIER2 also serves as a tool for drug screening, aiming to identify compounds that modulate its activity for therapeutic purposes. Overall, MIER2 represents a promising yet complex target in understanding transcriptional regulation and disease pathogenesis.
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