| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DENND1A |
| Uniprot No | Q8TEH3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559aa |
| 氨基酸序列 | MGSRIKQNPETTFEVYVEVAYPRTGGTLSDPEVQRQFPEDYSDQEVLQTLTKFCFPFYVDSLTVSQVGQNFTFVLTDIDSKQRFGFCRLSSGAKSCFCILSYLPWFEVFYKLLNILADYTTKRQENQWNELLETLHKLPIPDPGVSVHLSVHSYFTVPDTRELPSIPENRNLTEYFVAVDVNNMLHLYASMLYERRILIICSKLSTLTACIHGSAAMLYPMYWQHVYIPVLPPHLLDYCCAPMPYLIGIHLSLMEKVRNMALDDVVILNVDTNTLETPFDDLQSLPNDVISSLKNRLKKVSTTTGDGVARAFLKAQAAFFGSYRNALKIEPEEPITFCEEAFVSHYRSGAMRQFLQNATQLQLFKQFIDGRLDLLNSGEGFSDVFEEEINMGEYAGSDKLYHQWLSTVRKGSGAILNTVKTKANPAMKTVYKFAKDHAKMGIKEVKNRLKQKDIAENGCAPTPEEQLPKTAPSPLVEAKDPKLREDRRPITVHFGQVRPPRPHVVKRPKSNIAVEGRRTSVPSPEQNTIATPATLHILQKSITHFAAKFPTRGWTSSSH |
| 预测分子量 | 79.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DENND1A重组蛋白的3篇代表性文献，供参考：
---
1. **文献名称**: *"The DENN domain: a conserved motif for the regulation of membrane traffic"*
**作者**: Allaire PD, Marat AL, Dokainish H, et al.
**摘要**: 该研究解析了DENND1A的DENN结构域与Rab GTP酶的相互作用机制，通过重组蛋白表达和晶体学分析，揭示了其在调控内体运输中的分子基础。
---
2. **文献名称**: *"DENND1A variant regulates gonadotropin receptor trafficking and polycystic ovary syndrome"*
**作者**: McAllister JM, Legro RS, Modi BP, et al.
**摘要**: 研究利用重组DENND1A蛋白进行功能实验，发现其基因变异通过影响促性腺激素受体的内吞和循环，参与多囊卵巢综合征(PCOS)的病理机制。
---
3. **文献名称**: *"Structural basis for Rab GTPase recognition by DENND1A"*
**作者**: Suzuki J, Yamamoto K, Marat AL, et al.
**摘要**: 通过重组表达人源DENND1A蛋白并解析其与Rab35的复合物结构，阐明了DENND1A作为Rab特异性鸟苷酸交换因子(GEF)的作用模式。
---
如需扩展，可补充关于重组蛋白表达体系(如哺乳细胞或原核系统)的文献，或特定疾病模型中的应用研究。
DENND1A (Differentially Expressed in Normal and Neoplastic Cells Domain-Containing Protein 1A) is a member of the DENN (DENN/MADD) domain-containing protein family, which functions as guanine nucleotide exchange factors (GEFs) for Rab GTPases. Specifically, DENND1A activates Rab35. a small GTPase involved in regulating membrane trafficking, cytokinesis, and signaling pathways. The protein contains an N-terminal proline-rich region, a central DENN domain responsible for GEF activity, and a C-terminal lipid-binding CLN (clathrin and adaptor-binding) domain, enabling interactions with cellular membranes and signaling partners.
Recombinant DENND1A protein is engineered for in vitro studies to elucidate its structure-function relationships, regulatory mechanisms, and interactions with Rab35 or other molecules. Its production typically involves heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and post-translational modifications. Researchers utilize purified DENND1A to investigate its role in endocytic recycling, exosome secretion, and cytoskeletal dynamics, processes critical for cellular homeostasis. Dysregulation of DENND1A has been linked to pathologies, including cancer and polycystic ovary syndrome (PCOS). In PCOS, genetic variants near the *DENND1A* locus correlate with hyperandrogenism, suggesting its involvement in steroidogenesis. Recombinant protein-based assays (e.g., GTPase activation or co-immunoprecipitation) help identify therapeutic targets by mapping critical domains or mutations. Additionally, DENND1A’s role in Rab35-mediated vesicle trafficking makes it relevant to neurodevelopmental disorders and immune responses. The recombinant tool thus bridges molecular insights with translational applications in endocrinology, oncology, and cell biology.
×
