| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | STK32A |
| Uniprot No | Q8WU08 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-166aa |
| 氨基酸序列 | MGANTSRKPPVFDENEDVNFDHFEILRAIGKGSFGKVCIVQKNDTKKMYAMKYMNKQKCVERNEVRNVFKELQIMQGLEHPFLVNLWYSFQDEEDMFMVVDLLLGGDLRYHLQQNVHFKEETVKLFICELVMALDYLQNQRIIHRDMKPDNILLDEHDTWLSYKSH |
| 预测分子量 | 46.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于STK32A重组蛋白的参考文献示例(请注意，这些文献为假设性示例，实际文献需通过学术数据库查询)：
---
1. **文献名称**: *"Expression and Functional Characterization of Recombinant STK32A in Mammalian Cells"*
**作者**: Smith J, et al.
**摘要**: 本研究报道了STK32A重组蛋白在HEK293细胞中的表达与纯化，并通过体外激酶实验证实其具有磷酸化底物的活性，为进一步研究其在细胞信号通路中的作用提供了工具。
2. **文献名称**: *"Structural Insights into STK32A Kinase Domain via Recombinant Protein Crystallography"*
**作者**: Lee H, et al.
**摘要**: 通过大肠杆菌系统表达并纯化STK32A激酶结构域重组蛋白，利用X射线晶体学解析其三维结构，揭示了其ATP结合口袋的特征，为靶向药物设计奠定基础。
3. **文献名称**: *"STK32A Recombinant Protein Modulates Neuronal Differentiation in vitro"*
**作者**: Garcia R, et al.
**摘要**: 研究证明，重组STK32A蛋白能够通过激活MAPK通路促进神经干细胞的分化，提示其在神经发育中的潜在调控作用。
4. **文献名称**: *"Development of a High-Throughput Assay for STK32A Kinase Activity Using Recombinant Protein"*
**作者**: Chen L, et al.
**摘要**: 本文建立了一种基于荧光共振能量转移(FRET)的高通量检测方法，利用重组STK32A蛋白筛选小分子抑制剂，为相关疾病治疗提供候选化合物。
---
**建议**：以上内容为模拟示例。实际研究中，请通过**PubMed**、**Web of Science**或**Google Scholar**等平台，以关键词“STK32A recombinant protein”“STK32A kinase activity”检索真实文献。若研究较少，可扩展至STK家族其他成员或相关功能通路的研究。
STK32A (Serine/Threonine Kinase 32A) is a member of the serine/threonine kinase family, which plays roles in cellular signaling pathways regulating processes like proliferation, differentiation, and apoptosis. Although less characterized than other kinases, STK32A has drawn attention due to its potential involvement in developmental and pathological conditions. Studies suggest it may influence cerebellar development and neuronal functions, with genetic variants linked to neurological disorders. Its expression patterns in tissues like the brain, testes, and kidneys hint at diverse physiological roles.
Recombinant STK32A protein is engineered in vitro using expression systems (e.g., E. coli, mammalian cells) to produce purified, functional kinase for research. This tool enables mechanistic studies, such as mapping enzymatic activity, substrate interactions, and regulatory domains. Researchers utilize it to investigate phosphorylation events, screen kinase inhibitors, or validate antibodies. Epitope tags (e.g., His, FLAG) are often incorporated to facilitate purification and detection. Challenges include preserving post-translational modifications, which may require eukaryotic expression systems. Current efforts focus on elucidating STK32A’s signaling networks and its implications in diseases like cancer or neurodegeneration. Its recombinant form accelerates target validation and drug discovery, bridging gaps between genomic data and therapeutic applications.
×
