| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3GL1 |
| Uniprot No | Q99961 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-368aa |
| 氨基酸序列 | MSVAGLKKQFYKASQLVSEKVGGAEGTKLDDDFKEMEKKVDVTSKAVTEVLARTIEYLQPNPASRAKLTMLNTVSKIRGQVKNPGYPQSEGLLGECMIRHGKELGGESNFGDALLDAGESMKRLAEVKDSLDIEVKQNFIDPLQNLCEKDLKEIQHHLKKLEGRRLDFDYKKKRQGKIPDEELRQALEKFEESKEVAETSMHNLLETDIEQVSQLSALVDAQLDYHRQAVQILDELAEKLKRRMREASSRPKREYKPKPREPFDLGEPEQSNGGFPCTTAPKIAASSSFRSSDKPIRTPSRSMPPLDQPSCKALYDFEPENDGELGFHEGDVITLTNQIDENWYEGMLDGQSGFFPLSYVEVLVPLPQ |
| 预测分子量 | 45.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SH3GL1重组蛋白的3-4条参考文献示例(内容基于合理推测，建议通过学术数据库验证准确性)：
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1. **文献名称**：*Structural insights into SH3GL1-mediated membrane curvature sensing*
**作者**：Schmidt, A., et al.
**摘要**：通过重组表达SH3GL1蛋白的SH3结构域，结合X射线晶体学分析，揭示了其与dynamin-1的脯氨酸富集区互作机制，阐明其在调控突触小泡膜弯曲和内吞中的关键作用。
2. **文献名称**：*SH3GL1 regulates EGFR endocytosis through recombinant protein interaction studies*
**作者**：Wang, X., et al.
**摘要**：利用大肠杆菌表达的重组SH3GL1蛋白进行体外结合实验，证实其直接结合表皮生长因子受体(EGFR)的胞内结构域，调控受体内化及下游信号转导。
3. **文献名称**：*Recombinant SH3GL1 suppresses tumor growth by modulating apoptotic pathways*
**作者**：Li, H., et al.
**摘要**：研究发现，纯化的SH3GL1重组蛋白通过激活Bax/Bak依赖的线粒体凋亡通路，抑制癌细胞增殖，为靶向肿瘤治疗提供了实验依据。
4. **文献名称**：*SH3GL1 interacts with viral budding proteins via its N-terminal domain*
**作者**：Sundquist, W.I., et al.
**摘要**：通过重组蛋白的体外互作实验，证明SH3GL1的N端结构域与HIV-1病毒Gag蛋白结合，可能通过ESCRT复合物参与病毒颗粒出芽过程。
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**注意**：以上文献为模拟示例，实际引用时请通过PubMed、Google Scholar等平台核对作者、标题及摘要内容。
SH3GL1 (SH3 domain-containing GRB2-like protein 1), also known as EEN-B1 or CD3BP1. is a member of the SH3 domain-containing protein family. It plays a critical role in intracellular signaling, membrane trafficking, and cytoskeletal organization. The protein is characterized by an N-terminal coiled-coil domain and a C-terminal SH3 domain, which mediates protein-protein interactions by binding to proline-rich motifs in partner molecules. SH3GL1 is particularly implicated in endocytosis and vesicle trafficking processes, often interacting with components of the endocytic machinery such as dynamin and synaptojanin.
Functionally, SH3GL1 is involved in clathrin-mediated endocytosis, where it aids in recruiting and stabilizing dynamin at the necks of budding vesicles, facilitating membrane scission. It also participates in regulating receptor tyrosine kinase (RTK) signaling by modulating the internalization and degradation of activated receptors, thus influencing cell proliferation and differentiation pathways. Additionally, SH3GL1 has been linked to neuronal synaptic vesicle recycling, highlighting its importance in neurotransmission and brain function.
Recombinant SH3GL1 protein is engineered for in vitro studies to dissect its biochemical interactions, structural properties, and regulatory mechanisms. Produced using bacterial or mammalian expression systems, it retains functional domains necessary for binding assays, structural analyses (e.g., X-ray crystallography), or screening for inhibitors. Research utilizing SH3GL1 recombinant protein has contributed to understanding its role in diseases, including cancer (e.g., altered trafficking in tumorigenesis) and neurological disorders (e.g., synaptic dysfunction). Its study also offers insights into viral pathogenesis, as some pathogens exploit endocytic pathways involving SH3GL1 for host cell entry.
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