| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLXNA4 |
| Uniprot No | Q9HCM2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-521aa |
| 氨基酸序列 | LLTRQPAPLSQKQRSFVTFRGEPAEGFNHLVVDERTGHIYLGAVNRIYKLSSDLKVLVTHETGPDEDNPKCYPPRIVQTCNEPLTTTNNVNKMLLIDYKENRLIACGSLYQGICKLLRLEDLFKLGEPYHKKEHYLSGVNESGSVFGVIVSYSNLDDKLFIATAVDGKPEYFPTISSRKLTKNSEADGMFAYVFHDEFVASMIKIPSDTFTIIPDFDIYYVYGFSSGNFVYFLTLQPEMVSPPGSTTKEQVYTSKLVRLCKEDTAFNSYVEVPIGCERSGVEYRLLQAAYLSKAGAVLGRTLGVHPDDDLLFTVFSKGQKRKMKSLDESALCIFILKQINDRIKERLQSCYRGEGTLDLAWLKVKDIPCSSALLTIDDNFCGLDMNAPLGVSDMVRGIPVFTEDRDRMTSVIAYVYKNHSLAFVGTKSGKLKKSFGTGPQGGITQEWIGVEGDPPGANIASQEQMLCVYLQCSSHKAISDQRVQPLLCCFLNVPGNS |
| 预测分子量 | 60.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PLXNA4重组蛋白的参考文献(文献信息为模拟创作，供参考)：
1. **标题**: "Recombinant PLXNA4 extracellular domain production in HEK293 cells and its role in axon guidance"
**作者**: Takahashi H, et al.
**摘要**: 本研究利用HEK293细胞表达PLXNA4胞外结构域重组蛋白，验证其与Semaphorin配体的结合活性，并证明其参与神经元轴突导向的体外抑制作用。
2. **标题**: "Purification and structural analysis of PLXNA4 for cancer immunotherapy studies"
**作者**: Chen L, Wang Y.
**摘要**: 通过大肠杆菌表达系统制备重组PLXNA4蛋白，经亲和层析纯化后进行质谱鉴定，发现其与肿瘤微环境中T细胞迁移抑制相关，可能作为免疫治疗靶点。
3. **标题**: "PLXNA4-Fc融合蛋白抑制神经炎症的机制研究"
**作者**: Zhang R, et al.
**摘要**: 构建PLXNA4-Fc重组融合蛋白，在小鼠模型中证明其通过阻断Semaphorin 3A信号通路减轻多发性硬化症相关的神经炎症反应。
注：上述文献为根据领域研究热点生成的模拟内容，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词 "PLXNA4 recombinant protein expression" 或 "Plexin A4 structural/functional study" 查找真实文献。
**Background of PLXNA4 Recombinant Protein**
Plexin A4 (PLXNA4) is a transmembrane protein belonging to the plexin family, which serves as receptors for semaphorins, a class of signaling molecules critical for axon guidance, cell migration, and immune regulation. Structurally, PLXNA4 contains a large extracellular domain with semaphorin-binding motifs, a transmembrane region, and a cytoplasmic domain that mediates intracellular signaling. It plays a pivotal role in neurodevelopment by regulating neuronal connectivity, synaptic plasticity, and neural circuit formation through interactions with semaphorins like SEMA3A and SEMA6A. Beyond the nervous system, PLXNA4 is implicated in vascular remodeling, immune responses, and cancer progression, where it influences cell adhesion, invasion, and metastasis.
The recombinant PLXNA4 protein is engineered *in vitro* using expression systems (e.g., mammalian or insect cells) to produce purified, functional forms of the protein for research and therapeutic applications. This recombinant approach ensures high specificity and activity, enabling studies on PLXNA4’s structure-function relationships, ligand-receptor interactions, and downstream signaling pathways. Researchers utilize PLXNA4 recombinant proteins to investigate its role in diseases such as neurodegenerative disorders, autoimmune conditions, and malignancies. For instance, dysregulated PLXNA4 signaling has been linked to Alzheimer’s disease, multiple sclerosis, and tumor angiogenesis, making it a potential biomarker or therapeutic target.
In drug discovery, PLXNA4 recombinant proteins aid in screening inhibitors or agonists to modulate its activity. Their applications extend to structural biology (e.g., crystallography) and antibody development. Overall, PLXNA4 recombinant proteins are vital tools for unraveling its biological significance and advancing translational research in diverse pathologies.
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