| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNL3L |
| Uniprot No | Q9NVN8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-582aa |
| 氨基酸序列 | MMKLRHKNKKPGEGSKGHKKISWPYPQPAKQNGKKATSKVPSAPHFVHPNDHANREAELKKKWVEEMREKQQAAREQERQKRRTIESYCQDVLRRQEEFEHKEEVLQELNMFPQLDDEATRKAYYKEFRKVVEYSDVILEVLDARDPLGCRCFQMEEAVLRAQGNKKLVLVLNKIDLVPKEVVEKWLDYLRNELPTVAFKASTQHQVKNLNRCSVPVDQASESLLKSKACFGAENLMRVLGNYCRLGEVRTHIRVGVVGLPNVGKSSLINSLKRSRACSVGAVPGITKFMQEVYLDKFIRLLDAPGIVPGPNSEVGTILRNCVHVQKLADPVTPVETILQRCNLEEISNYYGVSGFQTTEHFLTAVAHRLGKKKKGGLYSQEQAAKAVLADWVSGKISFYIPPPATHTLPTHLSAEIVKEMTEVFDIEDTEQANEDTMECLATGESDELLGDTDPLEMEIKLLHSPMTKIADAIENKTTVYKIGDLTGYCTNPNRHQMGWAKRNVDHRPKSNSMVDVCSVDRRSVLQRIMETDPLQQGQALASALKNKKKMQKRADKIASKLSDSMMSALDLSGNADDGVGD |
| 预测分子量 | 81.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNL3L重组蛋白的模拟参考文献示例(文献为虚构，供参考)：
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1. **《Expression and Functional Analysis of Recombinant GNL3L in Cancer Cell Proliferation》**
*作者：Zhang Y, Li H, Wang X*
摘要：研究通过大肠杆菌系统成功表达并纯化GNL3L重组蛋白，发现其GTP酶活性对肝癌细胞HepG2的增殖具有显著调控作用，提示其在肿瘤发生中的潜在功能。
2. **《GNL3L Interacts with Telomerase RNA: A Study Based on Recombinant Protein Technology》**
*作者：Chen L, Smith JR, Kumar P*
摘要：利用哺乳动物细胞表达系统获得GNL3L重组蛋白，通过免疫共沉淀实验证实其与端粒酶RNA组分(TERC)的相互作用，为GNL3L参与端粒维持机制提供了证据。
3. **《Structural Characterization of GNL3L and Its Role in Ribosome Biogenesis》**
*作者：Tanaka M, Park S, Kim J*
摘要：通过X射线晶体学解析GNL3L重组蛋白结构，揭示其保守GTP结合域对核糖体RNA加工的关键作用，为核仁蛋白功能研究提供新视角。
4. **《Recombinant GNL3L Induces Apoptosis Resistance via PI3K/AKT Pathway》**
*作者：Gupta A, Liu T, Rao Z*
摘要：研究显示，过表达GNL3L重组蛋白通过激活PI3K/AKT信号通路抑制肺癌细胞凋亡，提示其可能成为癌症治疗的潜在靶点。
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注：以上文献为模拟生成，实际研究中建议通过PubMed或Web of Science等平台检索真实发表论文。
**Background of GNL3L Recombinant Protein**
GNL3L (G protein nucleolar 3-like) is a nucleolar GTP-binding protein belonging to the YlqF/YawG GTPase family, which shares structural and functional similarities with nucleostemin (GNL3) and its homologs. It is predominantly expressed in stem cells, cancer cells, and actively proliferating tissues, suggesting a role in cell cycle regulation, ribosome biogenesis, and maintenance of nucleolar integrity. GNL3L is implicated in diverse cellular processes, including ribosomal RNA (rRNA) processing, stress response, and telomere homeostasis. Its dysregulation has been linked to cancers, neurodegenerative disorders, and aging-related pathologies.
The recombinant GNL3L protein is engineered using *in vitro* expression systems (e.g., *E. coli* or mammalian cell lines) to produce a purified, biologically active form for functional studies. Recombinant technology enables precise control over protein structure, post-translational modifications, and scalability. GNL3L's GTPase activity, mediated by its conserved G-domain, is critical for its interaction with nucleolar components like B23 (nucleophosmin) and its role in rRNA maturation. Studies using recombinant GNL3L have elucidated its ability to bind GTP/GDP, regulate ribosome assembly, and influence cell proliferation via p53 and MDM2 pathways.
Research on GNL3L recombinant protein is pivotal for exploring its therapeutic potential. For instance, inhibiting GNL3L in cancer models reduces tumor growth, highlighting its oncogenic role. Conversely, its neuroprotective functions in neuronal stem cells underscore its dual context-dependent roles. Structural analyses of recombinant GNL3L have also identified nuclear localization signals and domains essential for nucleolar retention. Overall, GNL3L recombinant protein serves as a vital tool for dissecting its molecular mechanisms and developing targeted interventions in disease models.
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