| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMURF2 |
| Uniprot No | Q9HAU4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-748aa |
| 氨基酸序列 | MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGSGQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSNAINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLPDGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPISGTNGATCGQSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGVSTWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANLHLVLNRQNQLKDQQQQQVVSLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGHCRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEMLNPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPFYKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKPNGKSIPVNEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELELIICGLGKIDVNDWKVNTRLKHCTPDSNIVKWFWKAVEFFDEERRARLLQFVTGSSRVPLQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIEETCGFAVE |
| 预测分子量 | 88.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SMURF2重组蛋白的参考文献示例(内容基于研究领域常见方向，建议通过学术数据库核实具体文献)：
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1. **文献名称**：*"SMURF2 regulates TGF-β signaling by targeting the receptor for degradation"*
**作者**：Zhang, Y. et al.
**摘要**：本研究利用重组SMURF2蛋白，揭示了其通过泛素化修饰TGF-β受体并促进其蛋白酶体降解，从而负调控TGF-β信号通路，抑制细胞纤维化过程的机制。
2. **文献名称**：*"Structural insights into SMURF2-mediated ubiquitination in cancer metastasis"*
**作者**：Kavsak, P. et al.
**摘要**：通过体外表达SMURF2重组蛋白，作者解析了其与底物SMAD7的相互作用结构，证明SMURF2通过靶向肿瘤抑制因子促进泛素化依赖的降解，加剧肿瘤转移。
3. **文献名称**：*"SMURF2 recombinant protein modulates BMP signaling in osteogenesis"*
**作者**：Yamaguchi, K. et al.
**摘要**：研究发现，重组SMURF2蛋白通过泛素化BMP受体，负调控骨形态发生蛋白(BMP)信号通路，抑制成骨细胞分化，为骨代谢疾病提供了潜在治疗靶点。
4. **文献名称**：*"Engineering a stabilized SMURF2 recombinant protein for functional studies"*
**作者**：Wiesner, S. et al.
**摘要**：该文献报道了一种高稳定性SMURF2重组蛋白的制备方法，并验证其在体外泛素化实验中的活性，为研究SMURF2的酶活性和药物筛选提供了工具。
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**建议**：以上为示例文献，实际引用时请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 搜索关键词(如“SMURF2 recombinant protein”、“SMURF2 ubiquitin ligase”)获取最新及准确的文献信息。
SMURF2 (SMAD-specific E3 ubiquitin protein ligase 2) is a member of the HECT (homologous to E6-AP carboxyl terminus) family of ubiquitin ligases, playing a critical role in post-translational protein regulation via the ubiquitin-proteasome system. It mediates the targeted degradation of substrate proteins by attaching ubiquitin molecules, marking them for proteasomal destruction. Structurally, SMURF2 contains an N-terminal C2 domain for membrane interaction, a central WW domain for protein-protein interactions, and a C-terminal HECT domain responsible for ubiquitin transfer activity.
Primarily known for regulating TGF-β (transforming growth factor-beta) and BMP (bone morphogenetic protein) signaling pathways, SMURF2 ubiquitinates key components such as SMADs (SMAD1/5/7) and receptors, modulating cellular processes like differentiation, proliferation, and apoptosis. Its activity is tightly controlled through phosphorylation, subcellular localization, and interactions with adaptor proteins.
In disease contexts, SMURF2 exhibits dual roles. It can act as a tumor suppressor by degrading oncogenic substrates (e.g., TGF-β receptors) or promote cancer progression via destabilizing tumor suppressors (e.g., SMAD7). Dysregulation of SMURF2 is linked to fibrosis, osteoporosis, and metastatic cancers. Recombinant SMURF2 proteins, produced using bacterial or mammalian expression systems, are essential tools for studying ubiquitination mechanisms, screening inhibitors, and exploring therapeutic strategies. Researchers utilize these purified proteins in vitro to dissect enzymatic kinetics, substrate specificity, and structural interactions, aiding drug development for SMURF2-associated pathologies.
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