| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAP30BP |
| Uniprot No | Q9UHR5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 91-308aa |
| 氨基酸序列 | TEAEKRDPQELVASFSERVRNMSPDEIKIPPEPPGRCSNHLQDKIQKLYERKIKEGMDMNYIIQRKKEFRNPSIYEKLIQFCAIDELGTNYPKDMFDPHGWSEDSYYEALAKAQKIEMDKLEKAKKERTKIEFVTGTKKGTTTNATSTTTTTASTAVADAQKRKSKWDSAIPVTTIAQPTILTTTATLPAVVTVTTSASGSKTTVISAVGTIVKKAKQ |
| 预测分子量 | 28.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAP30BP重组蛋白的假设性参考文献示例(仅供参考，具体文献需根据实际研究查询)：
1. **《SAP30BP interacts with the SIN3/HDAC complex and modulates transcriptional repression》**
*作者：Zhang Y, et al.*
摘要：研究通过重组SAP30BP蛋白，验证其与SIN3/HDAC复合物的直接结合，揭示其在组蛋白去乙酰化介导的基因沉默中的调控作用。
2. **《Recombinant SAP30BP characterization and its role in chromatin remodeling》**
*作者：Wang L, et al.*
摘要：利用大肠杆菌系统表达并纯化SAP30BP重组蛋白，证明其通过结合组蛋白H3促进染色质凝集，为体外功能研究提供基础。
3. **《Structural analysis of SAP30BP reveals conserved functional domains》**
*作者：Kumar R, et al.*
摘要：通过X射线晶体学解析重组SAP30BP蛋白结构，鉴定其关键结合域，为靶向转录调控的药物开发提供结构依据。
4. **《SAP30BP overexpression enhances oncogenic pathways in breast cancer cells》**
*作者：Chen X, et al.*
摘要：利用重组SAP30BP蛋白进行功能增益实验，发现其通过调控Wnt信号通路促进肿瘤细胞增殖，提示潜在治疗靶点。
注：以上内容为模拟示例，实际文献需通过学术数据库(如PubMed、Web of Science)以关键词"SAP30BP recombinant protein"检索确认。
**Background of SAP30BP Recombinant Protein**
SAP30BP (SIN3A-associated protein 30 binding protein) is a conserved nuclear protein implicated in transcriptional regulation and chromatin remodeling. It belongs to the SAP30 family, which interacts with the SIN3/histone deacetylase (HDAC) corepressor complex, a key player in gene silencing and epigenetic modulation. SAP30BP shares homology with SAP30. a component of the SIN3/HDAC complex, and both proteins are thought to contribute to transcriptional repression by recruiting chromatin-modifying enzymes to specific genomic loci.
Structurally, SAP30BP contains a conserved N-terminal domain critical for binding to SIN3A and HDACs, as well as a C-terminal region involved in protein-protein interactions. Its recombinant form is typically produced in *E. coli* or mammalian expression systems, often fused with affinity tags (e.g., His-tag) for purification. Recombinant SAP30BP enables functional studies to dissect its role in gene regulation, particularly in contexts like cancer, where aberrant epigenetic silencing is common.
Functionally, SAP30BP is linked to cellular processes such as differentiation, proliferation, and apoptosis. Studies suggest it acts as a scaffold to stabilize SIN3/HDAC complexes on target promoters, enhancing deacetylation of histones and compacting chromatin to suppress transcription. Dysregulation of SAP30BP has been associated with tumorigenesis, as altered expression may disrupt normal gene silencing, promoting oncogene activation or tumor suppressor inactivation.
Research applications of recombinant SAP30BP include *in vitro* binding assays, chromatin immunoprecipitation (ChIP), and mechanistic studies of transcriptional repression. It also serves as a tool for exploring therapeutic strategies targeting epigenetic dysregulation in diseases like cancer. Further investigation is needed to fully elucidate its tissue-specific roles and interactions within broader regulatory networks.
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