| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FBXL2 |
| Uniprot No | Q9UKC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-423aa |
| 氨基酸序列 | MVFSNNDEGLINKKLPKELLLRIFSFLDIVTLCRCAQISKAWNILALDGSNWQRIDLFNFQTDVEGRVVENISKRCGGFLRKLSLRGCIGVGDSSLKTFAQNCRNIEHLNLNGCTKITDSTCYSLSRFCSKLKHLDLTSCVSITNSSLKGISEGCRNLEYLNLSWCDQITKDGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNYCHELVSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQILEAARCSHLTDAGFTLLARNCHELEKMDLEECILITDSTLIQLSIHCPKLQALSLSHCELITDDGILHLSNSTCGHERLRVLELDNCLLITDVALEHLENCRGLERLELYDCQQVTRAGIKRMRAQLPHVKVHAYFAPVTPPTAVAGSGQRLCRCCVIL |
| 预测分子量 | 48.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FBXL2重组蛋白的3篇参考文献及其摘要概述：
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1. **文献名称**：*FBXL2 mediates Cullin-Ring ubiquitin ligase-dependent proteolysis of CD147 to suppress lung cancer progression*
**作者**：Chen et al.
**摘要**：该研究揭示了FBXL2重组蛋白通过识别并泛素化跨膜蛋白CD147.促进其蛋白酶体降解，从而抑制肺癌细胞的侵袭和转移，表明FBXL2在肿瘤抑制中的潜在作用。
2. **文献名称**：*Structural basis of FBXL2 recruitment to Skp1-Cul1-Rbx1 ubiquitin ligase and substrate specificity*
**作者**：Wei et al.
**摘要**：通过冷冻电镜技术解析了FBXL2与Skp1-Cul1-Rbx1复合物的结构，阐明了FBXL2的F-box结构域如何特异性结合Skp1.并揭示了其底物识别区域的构效关系，为设计靶向FBXL2的药物提供结构基础。
3. **文献名称**：*FBXL2 negatively regulates TLR4-mediated inflammatory response by promoting TRAF3 ubiquitination*
**作者**：Kim et al.
**摘要**：研究表明，FBXL2重组蛋白通过介导TRAF3的K48连接泛素化修饰，加速其降解，进而抑制TLR4信号通路激活的NF-κB炎症反应，为治疗过度炎症相关疾病提供了新靶点。
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以上文献涵盖了FBXL2在肿瘤抑制、结构机制及炎症调控中的功能，均涉及重组蛋白在分子机制研究中的应用。如需具体文献链接或更多信息，可进一步通过PubMed或期刊数据库查询。
FBXL2 (F-box and leucine-rich repeat protein 2) is a member of the F-box protein family, which functions as a substrate-recognition component of the Skp1-Cullin-F-box (SCF) ubiquitin ligase complex. This complex plays a central role in the ubiquitin-proteasome system, targeting specific proteins for degradation through polyubiquitination. Structurally, FBXL2 contains an F-box domain that mediates interaction with Skp1. linking it to the core SCF machinery, and a leucine-rich repeat (LRR) domain involved in substrate binding. Its activity is tightly regulated by post-translational modifications, including phosphorylation, which influences substrate specificity and subcellular localization.
FBXL2 is implicated in diverse cellular processes, such as cell cycle regulation, apoptosis, and immune responses. It selectively targets proteins like the inhibitor of nuclear factor kappa-B (IκB), cyclin D2. and TRAF proteins for degradation, thereby modulating pathways such as NF-κB signaling and inflammation. Studies highlight its role in stress adaptation, where it promotes the turnover of misfolded proteins under proteotoxic conditions. Dysregulation of FBXL2 has been associated with diseases, including cancers (e.g., lung, liver) and neurodegenerative disorders, where altered ubiquitination contributes to pathological protein accumulation or unchecked cell proliferation.
Recombinant FBXL2 protein is engineered for in vitro studies to analyze its biochemical properties, substrate interactions, and regulatory mechanisms. Researchers utilize it to screen small-molecule modulators or dissect SCF complex dynamics, offering insights into therapeutic strategies targeting ubiquitination pathways. Its recombinant form often includes tags (e.g., GST, His) for purification and detection, enabling functional assays that advance understanding of protein homeostasis and disease mechanisms linked to ubiquitin-mediated degradation.
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