| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | H2BC4 |
| Uniprot No | P62807 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-125aa |
| 氨基酸序列 | PEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSVYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSS |
| 预测分子量 | 40.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于H2BC4重组蛋白的参考文献示例(注：文献信息为模拟虚构，仅用于格式参考)：
1. **标题**：Recombinant H2BC4 Facilitates Nucleosome Assembly in vitro
**作者**：Smith J, et al.
**摘要**：研究报道了通过大肠杆菌表达系统高效纯化重组H2BC4蛋白，并验证其在体外促进核小体组装的能力，揭示了其C端结构域对DNA结合的调控作用。
2. **标题**：Structural Insights into H2BC4 Interaction with Histone Chaperones
**作者**：Zhang L, et al.
**摘要**：利用重组H2BC4蛋白进行晶体结构分析和分子对接实验，阐明其与组蛋白伴侣DAXX的相互作用机制，为染色质重塑机制提供新证据。
3. **标题**：H2BC4 Post-Translational Modifications Studied via Recombinant Protein Screening
**作者**：Tanaka K, et al.
**摘要**：开发基于重组H2BC4的体外修饰平台，鉴定出多个新型乙酰化和泛素化位点，探讨其在细胞周期调控中的潜在功能。
4. **标题**：Recombinant H2BC4 as a Tool for Epigenetic Drug Discovery
**作者**：Brown R, et al.
**摘要**：构建重组H2BC4高通量筛选模型，筛选出小分子化合物靶向H2B与组蛋白去乙酰化酶(HDAC)的相互作用，为抗癌药物开发提供新策略。
**注**：以上文献为示例性质，实际引用需通过学术数据库(如PubMed、Web of Science)检索真实发表的研究。
**Background of H2BC4 Recombinant Protein**
H2BC4. a member of the histone H2B family, is a core component of nucleosomes, the fundamental repeating units of eukaryotic chromatin. Histones play a critical role in packaging DNA into structural and functional units, regulating gene expression, and maintaining genome stability. The H2B family consists of multiple subtypes, with H2BC4 (also called H2B1C) encoded by the *H2BC4* gene in humans. It is involved in forming the nucleosome octamer by pairing with H2A, H3. and H4 histones, around which approximately 147 base pairs of DNA are wrapped.
Recombinant H2BC4 protein is produced using biotechnological methods, such as expression in bacterial or eukaryotic systems, followed by purification to ensure high homogeneity and biological activity. This engineered protein retains the functional properties of native H2BC4. enabling its use in studying chromatin dynamics, DNA repair mechanisms, and epigenetic regulation. Researchers employ recombinant H2BC4 in *in vitro* assays, including nucleosome reconstitution, histone modification studies (e.g., acetylation, methylation), and interactions with chromatin-associated proteins.
Interest in H2BC4 also stems from its potential roles in diseases. Dysregulation of histone function or expression is linked to cancers, developmental disorders, and autoimmune conditions. For instance, altered H2B subtypes may influence tumor progression by modulating oncogene or tumor suppressor activity. Recombinant H2BC4 facilitates mechanistic investigations into these processes, aiding drug discovery efforts targeting chromatin-related pathways. Additionally, it serves as a tool for developing antibodies or diagnostic markers.
Overall, recombinant H2BC4 is a vital resource for advancing our understanding of chromatin biology and its implications in health and disease. Its applications span basic research, therapeutic development, and biomedical diagnostics.
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