| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NELFB |
| Uniprot No | Q8WX92 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 8-199aa |
| 氨基酸序列 | LGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLHGTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPVVMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYILEKESALFSTELSVLHNFFSPSPKTRRQGE |
| 预测分子量 | 48.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NELFB重组蛋白的3篇示例参考文献(内容基于研究领域常见方向，部分信息为合理推测，建议通过学术数据库核实)：
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1. **标题**：*Structural insights into NELFB subunit of the human Negative Elongation Factor complex*
**作者**：Yamaguchi Y, et al.
**摘要**：通过X射线晶体学解析了重组NELFB蛋白的晶体结构，揭示了其与NELF-E亚基的相互作用界面，并探讨其在RNA聚合酶II转录暂停调控中的分子机制。
2. **标题**：*Functional characterization of recombinant NELFB in transcriptional regulation*
**作者**：Li S, et al.
**摘要**：在大肠杆菌中成功表达并纯化重组NELFB蛋白，通过体外生化实验证明其与RNA聚合酶II及DSIF复合体的协同作用，证实NELFB在基因启动子近端暂停中的关键功能。
3. **标题**：*NELFB knockdown disrupts oncogenic signaling in leukemia cells*
**作者**：Smith J, et al.
**摘要**：利用重组NELFB蛋白进行功能拯救实验，发现其缺失导致白血病细胞中MYC等癌基因异常激活，提示NELFB通过调控转录延伸维持基因组稳定性。
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**注**：以上文献为示例，实际研究中请通过PubMed或Google Scholar以关键词“NELFB recombinant protein”“NELF complex structure”等检索最新论文。
NELFB (Negative Elongation Factor B) is a critical subunit of the NELF complex (NELF-A, -B, -C/D), which regulates transcriptional pausing during early elongation by RNA polymerase II (Pol II). Discovered in the late 1990s, NELF works alongside the DRB sensitivity-inducing factor (DSIF) to stabilize paused Pol II at promoter-proximal regions, a key checkpoint in metazoan gene expression. This pausing mechanism allows precise control of transcriptional activation, enabling rapid responses to developmental or environmental signals. NELFB, encoded by the *WHSC2* gene, directly interacts with RNA and other NELF subunits to maintain the paused state. Its dysfunction is linked to cancers and neurodevelopmental disorders, highlighting its biological significance.
Recombinant NELFB protein, produced via bacterial or mammalian expression systems, is essential for *in vitro* studies of transcriptional regulation. Purification typically involves affinity tagging (e.g., His-tag) followed by chromatography. Researchers use recombinant NELFB to reconstitute the NELF complex, analyze DNA/RNA binding activities, and screen for inhibitors targeting transcriptional pausing in diseases like HIV (where NELF restricts viral latency) or cancer (where aberrant pausing escape drives oncogene expression). Recent CRISPR-based studies also employ recombinant NELFB to explore its non-canonical roles in chromatin remodeling and 3D genome organization. As a tool, it bridges structural biology and functional genomics, offering insights into Pol II dynamics and therapeutic strategies for transcription-dependent pathologies.
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