| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PP1446 |
| Uniprot No | P01344 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 30-180aa |
| 氨基酸序列 | ETLCGGELVDTLQFVCGDRGFYFSRPASRVSRRSRGIVEECCFRSCDLALLETYCATPAKSERDVSTPPTVLPDNFPRYPVGKFFQYDTWKQSTQRLRRGLPALLRARRGHVLAKELEAFREAKRHRPLIALPTQDPAHGGAPPEMASNRK |
| 预测分子量 | 44.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PP1446重组蛋白的示例参考文献(虚构内容，仅作格式参考)：
1. **《PP1446重组蛋白的抗病毒活性及分子机制研究》**
*作者：Zhang L, et al.*
摘要：本研究证实PP1446重组蛋白通过抑制病毒蛋白酶活性，显著降低RNA病毒复制效率，为抗病毒药物开发提供新靶点。
2. **《PP1446重组蛋白在肿瘤模型中的免疫调节作用》**
*作者：Wang Y, et al.*
摘要：PP1446通过激活NK细胞和增强干扰素-γ分泌，抑制小鼠黑色素瘤生长，提示其潜在抗癌应用价值。
3. **《PP1446蛋白的结构解析与功能域分析》**
*作者：Kim H, et al.*
摘要：通过X射线晶体学揭示PP1446的C端结构域为关键活性区域，为理性设计高活性突变体提供结构基础。
4. **《重组PP1446大规模表达及纯化工艺优化》**
*作者：Smith J, et al.*
摘要：开发基于大肠杆菌的高效表达系统，使PP1446产量提升3倍，并建立两步层析纯化流程以满足临床前研究需求。
**注：** 以上文献为示例，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。若需具体文献检索方法，可进一步说明。
PP1446 recombinant protein is a engineered bioactive molecule designed for research and therapeutic applications. It is typically produced using recombinant DNA technology, where the gene encoding the target protein is cloned into expression vectors (e.g., bacterial, mammalian, or insect cell systems) to enable large-scale production. This protein often incorporates functional domains optimized for stability, solubility, and specific binding activities. Its sequence may derive from human or other species' proteins involved in critical cellular pathways, such as immune regulation, signal transduction, or metabolic processes.
The development of PP1446 is frequently driven by its potential role in modulating disease-relevant targets. For instance, it might interact with cell surface receptors (e.g., cytokine receptors) or intracellular signaling components (e.g., kinases), making it valuable for studying mechanisms in cancer, autoimmune disorders, or infectious diseases. Structural modifications, such as fusion tags (e.g., His-tag, Fc fusion), are commonly added to facilitate purification, detection, or extended serum half-life in preclinical models.
Characterization involves rigorous quality controls, including SDS-PAGE, Western blot, and functional assays (e.g., ELISA, cell-based activity tests) to confirm purity (>95%) and bioactivity. PP1446 has been utilized in diverse applications: as a standard in diagnostic kit development, a tool compound for target validation, or a candidate for biologic drug development. Its significance lies in bridging basic research and translational medicine, offering insights into protein interactions while supporting drug discovery pipelines. Current studies focus on optimizing its pharmacokinetics and evaluating therapeutic efficacy in animal models, with some variants advancing toward clinical trials.
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