| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZMAT2 |
| Uniprot No | Q96NC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-194aa |
| 氨基酸序列 | MASGSGTKNLDFRRKWDKDEYEKLAEKRLTEEREKKDGKPVQPVKRELLRHRDYKVDLESKLGKTIVITKTTPQSEMGGYYCNVCDCVVKDSINFLDHINGKKHQRNLGMSMRVERSTLDQVKKRFEVNKKKMEEKQKDYDFEERMKELREEEEKAKAYKKEKQKEKKRRAEEDLTFEEDDEMAAVMGFSGFGS |
| 预测分子量 | 27.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZMAT2重组蛋白的模拟参考文献示例(注：以下内容为假设性示例，实际文献需通过学术数据库查询)：
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1. **文献名称**：*Structural and functional characterization of recombinant ZMAT2 in RNA splicing*
**作者**：Chen L, et al.
**摘要**：本研究通过大肠杆菌表达系统成功制备了重组ZMAT2蛋白，利用X射线晶体学解析其锌指结构域的三维结构，并证明其通过结合特定RNA序列调控剪接体组装。
2. **文献名称**：*ZMAT2 interacts with p53: Role of recombinant protein in apoptosis regulation*
**作者**：Wang Y, et al.
**摘要**：作者在HEK293细胞中重组表达并纯化ZMAT2蛋白，通过体外Pull-down实验验证其与肿瘤抑制蛋白p53的直接相互作用，提示ZMAT2可能通过p53通路参与细胞凋亡调控。
3. **文献名称**：*Optimization of ZMAT2 recombinant expression in mammalian cells for high-throughput screening*
**作者**：Kumar S, et al.
**摘要**：文章优化了ZMAT2在哺乳动物细胞中的重组表达条件，采用亲和层析和尺寸排阻色谱纯化，获得高纯度蛋白用于基于体外RNA结合活性的药物筛选平台开发。
4. **文献名称**：*ZMAT2 knockdown and recombinant rescue experiments reveal its essential role in ribosome biogenesis*
**作者**：Garcia-Ruiz H, et al.
**摘要**：通过siRNA敲低ZMAT2导致核糖体RNA加工异常，而外源添加重组ZMAT2蛋白可部分恢复表型，证明其在核糖体生物合成中的关键作用。
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**提示**：实际研究中，建议通过PubMed或Google Scholar以“ZMAT2 recombinant protein”、“ZMAT2 expression and purification”等关键词检索最新文献，并优先选择近5年内的结构生物学或功能机制研究。
**Background of ZMAT2 Recombinant Protein**
ZMAT2 (Zinc Finger Matrin-Type 2), also known as Wig1 (Wild-type p53-Induced Gene 1), is a nuclear protein encoded by the *ZMAT2* gene in humans. It belongs to the zinc finger protein family, characterized by matrin-type zinc finger domains involved in nucleic acid binding. ZMAT2 was initially identified as a transcriptional target of the tumor suppressor p53. playing a role in cellular stress responses, including apoptosis and cell cycle regulation. Studies suggest it binds to RNA and DNA, influencing RNA stability, splicing, or translation, though its precise molecular mechanisms remain under investigation.
Structurally, ZMAT2 contains two Cys2-His2-type zinc finger motifs critical for its interaction with nucleic acids. The recombinant ZMAT2 protein is typically produced using expression systems like *E. coli* or mammalian cell lines (e.g., HEK293), ensuring proper folding and post-translational modifications. Affinity tags (e.g., His-tag) are often incorporated to facilitate purification via chromatography.
Recombinant ZMAT2 is widely used in functional studies to explore its role in cancer biology, particularly its dual context-dependent pro-survival or pro-apoptotic effects. It has been implicated in diseases such as glioblastoma and hepatocellular carcinoma, where dysregulation correlates with tumor progression or chemoresistance. Additionally, recombinant ZMAT2 serves as a tool for analyzing protein-RNA/DNA interactions, screening therapeutic compounds, or generating antibodies.
Overall, ZMAT2 recombinant protein is a vital reagent for deciphering its biological functions and potential applications in diagnostics or targeted therapies.
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