| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | Avpr1a |
| Uniprot No | P37288 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-418aa |
| 氨基酸序列 | MRLSAGPDAGPSGNSSPWWPLATGAGNTSREAEALGEGNGPPRDVRNEELAKLEIAVLAVTFAVAVLGNSSVLLALHRTPRKTSRMHLFIRHLSLADLAVAFFQVLPQMCWDITYRFRGPDWLCRVVKHLQVFGMFASAYMLVVMTADRYIAVCHPLKTLQQPARRSRLMIAAAWVLSFVLSTPQYFVFSMIEVNNVTKARDCWATFIQPWGSRAYVTWMTGGIFVAPVVILGTCYGFICYNIWCNVRGKTASRQSKGAEQAGVAFQKGFLLAPCVSSVKSISRAKIRTVKMTFVIVTAYIVCWAPFFIIQMWSVWDPMSVWTESENPTITITALLGSLNSCCNPWIYMFFSGHLLQDCVQSFPCCQNMKEKFNKEDTDSMSRRQTFYSNNRSPTNSTGMWKDSPKSSKSIKFIPVST |
| 预测分子量 | 46,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于Avpr1a重组蛋白的3篇参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*Expression and Pharmacological Characterization of Recombinant Human AVPR1a in HEK293 Cells*
**作者**：Smith J, et al.
**摘要**：本研究在HEK293细胞中成功表达重组人源AVPR1a，通过放射性配体结合实验和cAMP检测验证其功能活性，证实其与精氨酸加压素(AVP)的高亲和力结合及下游信号传导能力，为受体药理学研究提供模型。
2. **文献名称**：*Structural Insights into AVPR1a Activation via Cryo-EM Analysis of the Recombinant Receptor*
**作者**：Zhang Y, et al.
**摘要**：利用昆虫细胞表达系统制备重组AVPR1a蛋白，结合冷冻电镜技术解析其与激动剂复合物的三维结构，揭示受体激活过程中跨膜结构域的构象变化，为靶向药物设计提供依据。
3. **文献名称**：*Optimized Bacterial Expression and Refolding of AVPR1a for High-Throughput Screening*
**作者**：Brown M, et al.
**摘要**：开发了一种在大肠杆菌中表达AVPR1a胞外域的方法，通过包涵体复性和亲和层析获得可溶性蛋白，并验证其在体外拮抗剂筛选中的实用性，显著降低研究成本。
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**备注**：以上文献为示例，实际引用时需根据具体研究内容检索真实发表的论文。
The arginine vasopressin receptor 1A (AVPR1A) is a G protein-coupled receptor (GPCR) that primarily mediates the physiological and behavioral effects of arginine vasopressin (AVP), a neuropeptide involved in regulating social behavior, stress response, and fluid homeostasis. AVPR1A is expressed in various tissues, including the brain, kidneys, and liver, and plays a critical role in modulating social bonding, aggression, and circadian rhythms. Dysregulation of AVPR1A signaling has been implicated in neuropsychiatric disorders such as autism spectrum disorder, anxiety, and depression.
Recombinant AVPR1A protein is engineered in vitro using expression systems like mammalian cells (e.g., HEK293 or CHO cells) to produce functional receptor proteins for research applications. This involves cloning the AVPR1A gene into a plasmid vector, transfecting host cells, and purifying the expressed protein via affinity chromatography or other methods. The recombinant form retains ligand-binding specificity and downstream signaling capabilities, enabling studies on receptor-ligand interactions, signal transduction pathways, and drug screening.
Researchers utilize recombinant AVPR1A to investigate its structural dynamics, including ligand-binding domains and conformational changes during activation. It also serves as a tool for developing therapeutics targeting AVPR1A-related disorders. Additionally, in vitro assays with the recombinant protein help elucidate the receptor’s role in cellular responses to AVP or synthetic analogs, providing insights into its physiological and pathological mechanisms. Its applications extend to high-throughput screening for agonists/antagonists and personalized medicine approaches in neuropharmacology.
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