| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FPGT |
| Uniprot No | O14772 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-594aa |
| 氨基酸序列 | MAAARDPPEVSLREATQRKLRRFSELRGKLVARGEFWDIVAITAADEKQELAYNQQLSEKLKRKELPLGVQYHVFVDPAGAKIGNGGSTLCALQCLEKLYGDKWNSFTILLIHSGGYSQRLPNASALGKIFTALPLGNPIYQMLELKLAMYIDFPLNMNPGILVTCADDIELYSIGEFEFIRFDKPGFTALAHPSSLTIGTTHGVFVLDPFDDLKHRDLEYRSCHRFLHKPSIEKMYQFNAVCRPGNFCQQDFAGGDIADLKLDSDYVYTDSLFYMDHKSAKMLLAFYEKIGTLSCEIDAYGDFLQALGPGATVEYTRNTSNVIKEESELVEMRQRIFHLLKGTSLNVVVLNNSKFYHIGTTEEYLFYFTSDNSLKSELGLQSITFSIFPDIPECSGKTSCIIQSILDSRCSVAPGSVVEYSRLGPDVSVGENCIISGSYILTKAALPAHSFVCSLSLKMNRCLKYATMAFGVQDNLKKSVKTLSDIKLLQFFGVCFLSCLDVWNLKVTEELFSGNKTCLSLWTARIFPVCSSLSDSVITSLKMLNAVKNKSAFSLNSYKLLSIEEMLIYKDVEDMITYREQIFLEISLKSSLM |
| 预测分子量 | 68.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FPGT重组蛋白的3篇参考文献示例：
1. **文献名称**：*Recombinant expression and functional characterization of human FPGT in mammalian cells*
**作者**：Smith, J., et al. (2015)
**摘要**：研究通过哺乳动物表达系统成功重组表达了人源FPGT蛋白，分析了其酶活性和底物特异性，揭示了其在岩藻糖基化修饰中的关键作用。
2. **文献名称**：*Structural insights into FPGT using recombinant protein crystallography*
**作者**：Zhang, L., et al. (2018)
**摘要**：通过重组FPGT蛋白的结晶与结构解析，阐明了其催化机制及与GDP-岩藻糖结合的活性位点，为靶向药物设计提供了结构基础。
3. **文献名称**：*FPGT knockout cell lines generated via CRISPR/Cas9 reveal glycosylation-dependent disease pathways*
**作者**：Chen, Y., et al. (2020)
**摘要**：利用重组FPGT蛋白回补实验，验证了FPGT缺失对细胞粘附蛋白糖基化的影响，并关联了其在癌症转移中的潜在调控机制。
(注：以上文献信息为示例，实际文献需根据具体研究通过数据库检索获取。)
Fructose-1-phosphate guanylyltransferase (FPGT), also known as GFPP synthase, is a conserved enzyme encoded by the *FPGT* gene in humans. It catalyzes the transfer of a guanylyl group from GTP to fructose-1-phosphate, forming GDP-fructose—a critical step in the biosynthesis of GDP-sugars involved in protein glycosylation pathways. This enzymatic activity links carbohydrate metabolism with post-translational modification processes, particularly O-linked glycosylation, which influences protein stability, cellular signaling, and intercellular communication.
The interest in recombinant FPGT protein stems from its role in studying congenital disorders of glycosylation (CDGs) and metabolic diseases. Mutations in *FPGT* have been associated with abnormal glycosylation patterns, leading to multisystemic clinical manifestations. Recombinant FPGT, typically produced in *E. coli* or mammalian expression systems, enables biochemical characterization of enzyme kinetics, substrate specificity, and inhibitor screening. Its purified form is essential for structural studies (e.g., X-ray crystallography) to elucidate catalytic mechanisms and design therapeutic interventions.
Recent research highlights FPGT's potential involvement in cancer and immune regulation, as altered glycosylation is a hallmark of malignant cells. Recombinant FPGT also serves as a tool to explore GDP-fructose's role in modulating Notch signaling and other developmental pathways. Pharmaceutical applications include developing small-molecule modulators to correct glycosylation defects or target FPGT-related pathways in disease contexts. Advances in recombinant protein technology have improved FPGT yield and stability, facilitating high-throughput assays and translational studies. Ongoing efforts focus on linking FPGT dysfunction to specific pathologies and optimizing its recombinant production for therapeutic protein engineering.
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