| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRT85 |
| Uniprot No | P78386 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-507aa |
| 氨基酸序列 | MSCRSYRISSGCGVTRNFSSCSAVAPKTGNRCCISAAPYRGVSCYRGLTGFGSRSLCNLGSCGPRIAVGGFRAGSCGRSFGYRSGGVCGPSPPCITTVSVNESLLTPLNLEIDPNAQCVKQEEKEQIKSLNSRFAAFIDKVRFLEQQNKLLETKWQFYQNQRCCESNLEPLFSGYIETLRREAECVEADSGRLASELNHVQEVLEGYKKKYEEEVALRATAENEFVVLKKDVDCAYLRKSDLEANVEALVEESSFLRRLYEEEIRVLQAHISDTSVIVKMDNSRDLNMDCIIAEIKAQYDDVASRSRAEAESWYRSKCEEMKATVIRHGETLRRTKEEINELNRMIQRLTAEIENAKCQRAKLEAAVAEAEQQGEAALSDARCKLAELEGALQKAKQDMACLLKEYQEVMNSKLGLDIEIATYRRLLEGEEHRLCEGVGSVNVCVSSSRGGVSCGGLSYSTTPGRQITSGPSAIGGSITVVAPDSCAPCQPRSSSFSCGSSRSVRFA |
| 预测分子量 | 57.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KRT85重组蛋白的3篇参考文献示例(内容基于研究领域常见方向，部分为假设性概括)：
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1. **文献名称**: *Structural characterization of recombinant human KRT85 and its role in hair follicle integrity*
**作者**: Smith J, Brown R, et al.
**摘要**: 本研究通过原核表达系统成功纯化了重组人KRT85蛋白，并利用X射线晶体学解析其结构。发现KRT85的螺旋结构域对维持毛囊角蛋白纤维的稳定性至关重要，突变分析揭示了其与遗传性毛发疾病(如单纯型大疱性表皮松解症)的相关性。
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2. **文献名称**: *In vitro assembly of KRT85-KRT35 heterodimers: implications for mechanical strength in keratinocytes*
**作者**: Chen L, Wang Y, et al.
**摘要**: 通过共表达KRT85(II型角蛋白)与KRT35(I型角蛋白)的重组蛋白，研究两者在体外形成异源二聚体的动态过程。实验表明，KRT85的C末端结构域对细胞骨架网络的形成和机械应力抵抗具有关键作用，为开发靶向角蛋白疾病的药物提供依据。
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3. **文献名称**: *Recombinant KRT85 as a potential biomarker for alopecia areata*
**作者**: Gupta S, Lee M, et al.
**摘要**: 利用哺乳动物细胞系表达重组KRT85蛋白，并检测其在斑秃患者血清中的自身抗体水平。结果显示，KRT85的异常免疫反应可能参与疾病发生，提示其作为诊断标志物的潜力。
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**备注**：以上文献为示例性内容，实际文献需通过PubMed、Web of Science等数据库检索确认。建议使用关键词“KRT85 recombinant”或“Keratin 85 expression”查找最新研究。
**Background of KRT85 Recombinant Protein**
KRT85. a member of the keratin family, is a type II intermediate filament protein encoded by the *KRT85* gene. Keratins are structural proteins critical for maintaining the integrity and mechanical resilience of epithelial cells, particularly in tissues like hair, nails, and skin. KRT85 is specifically expressed in hard keratinized structures, including the hair cortex and nail matrix, where it forms heteropolymers with type I keratins (e.g., KRT35) to create durable filaments. These filaments contribute to the tensile strength and elasticity of hair shafts, playing a vital role in hair growth and structural stability.
Mutations in *KRT85* are associated with hereditary hair disorders, such as monilethrix (characterized by fragile, beaded hair) and ectodermal dysplasia syndromes. Studying KRT85 helps elucidate the molecular basis of these conditions and informs potential therapeutic strategies. Recombinant KRT85 protein, produced via expression systems like *E. coli* or mammalian cells, enables in vitro studies of its biochemical properties, interactions with binding partners, and role in disease mechanisms.
In research, KRT85 recombinant protein is utilized to investigate hair follicle biology, develop cellular models of keratinopathies, and screen compounds targeting keratin-related pathologies. It also aids in producing antibodies for diagnostic applications. By providing a controlled, pure protein source, recombinant KRT85 facilitates structural analyses (e.g., X-ray crystallography) and functional assays, advancing our understanding of keratin networks in health and disease. This protein thus serves as a vital tool for both basic research and translational studies in dermatology and genetics.
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