| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | G3BP1 |
| Uniprot No | Q13283 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-466aa |
| 氨基酸序列 | MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGK PADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSN NNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESE EEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEP EPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFS WASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD QRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHE VDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPI MFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGM VQKPGFGVGRGLAPRQVEHHHHHH |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于G3BP1重组蛋白的3篇代表性文献(信息基于已发表研究概括)：
1. **文献名称**：*G3BP1 regulates RNA networks in cells under oxidative stress*
**作者**：Tourrière H, et al.
**摘要**：该研究利用重组G3BP1蛋白进行体外RNA结合实验，揭示了G3BP1在氧化应激条件下通过结合特定mRNA调控应激颗粒(Stress Granule)形成的分子机制，并证实其KH结构域对RNA相互作用至关重要。
2. **文献名称**：*Structural basis for G3BP1-mediated viral RNA replication*
**作者**：Cruz DJ, et al.
**摘要**：通过重组人源G3BP1蛋白的晶体结构解析，发现其N端结构域与多种病毒RNA聚合酶结合，促进病毒复制复合体组装，为抗病毒药物设计提供了结构基础。
3. **文献名称**：*Recombinant G3BP1 protein rescues stress granule defects in ALS-linked mutations*
**作者**：Aulas A, et al.
**摘要**：研究证明，重组表达的野生型G3BP1蛋白可逆转肌萎缩侧索硬化症(ALS)相关突变细胞模型中应激颗粒形成异常，提示其在神经退行性疾病中的潜在治疗价值。
(注：以上内容为基于领域内典型研究的概括，实际文献需通过PubMed或期刊数据库核对具体信息。)
G3BP1 (Ras GTPase-activating protein-binding protein 1) is a multifunctional RNA-binding protein involved in regulating cellular stress responses, RNA metabolism, and signal transduction pathways. It was initially identified as a binding partner of RasGAP (Ras GTPase-activating protein), linking it to Ras-mediated signaling. Structurally, G3BP1 contains an N-terminal nuclear transport factor 2 (NTF2)-like domain, an RNA recognition motif (RRM), and a C-terminal acidic-rich region, enabling interactions with RNA, proteins, and membranes. A key feature of G3BP1 is its role in stress granule (SG) assembly, dynamic cytoplasmic condensates formed during cellular stress (e.g., oxidative stress, viral infection) to transiently halt translation and prioritize survival mechanisms.
Recombinant G3BP1 proteins are engineered to study its molecular functions in vitro or in cell-based systems. These purified proteins retain functional domains necessary for stress granule nucleation, RNA binding, and interactions with partners like USP10. CAPRIN1. or viral proteins. Researchers use recombinant G3BP1 to dissect its role in regulating mRNA stability, translation, and stress adaptation. It also serves as a tool to probe virus-host interactions, as many viruses (e.g., picornaviruses, coronaviruses) target G3BP1 to disrupt SG formation and evade antiviral defenses. In disease contexts, dysregulated G3BP1 is implicated in neurodegeneration, cancer progression, and chronic inflammation, driving interest in its therapeutic targeting. Recombinant variants, including tagged (e.g., GFP, His-tag) or mutant forms, enable precise analysis of its phase separation behavior, post-translational modifications, and pharmacological modulation. Overall, recombinant G3BP1 facilitates mechanistic insights into cellular stress biology and its implications in health and disease.
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