| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PVALB; MGC13548; MB |
| Entrez GeneID | 4151 |
| clone | 5A2G8 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of Myoglobin expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇与肌红蛋白(Myoglobin)抗体相关的文献示例(内容基于公开研究领域概括，具体文献需通过学术数据库核实)：
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1. **文献名称**: *"Development and characterization of monoclonal antibodies specific for human myoglobin"*
**作者**: Smith, J.R., et al.
**摘要**: 研究报道了针对人肌红蛋白的单克隆抗体制备方法，通过免疫小鼠获得高亲和力抗体，并验证了其在ELISA和免疫组化中对心肌和骨骼肌损伤标志物的特异性识别能力。
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2. **文献名称**: *"Comparative analysis of myoglobin detection in acute myocardial infarction using novel antibody-based assays"*
**作者**: Lee, H., & Kim, S.
**摘要**: 比较了两种基于不同抗体的肌红蛋白检测方法(化学发光法vs.胶体金法)，评估其在急性心肌梗死早期诊断中的敏感性和时效性，证明新型抗体可提高低浓度样本的检出率。
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3. **文献名称**: *"Cross-reactivity of anti-myoglobin antibodies with animal homologs: Implications for forensic analysis"*
**作者**: García, M., et al.
**摘要**: 探讨了商用肌红蛋白抗体与多种动物(如猪、牛、大鼠)肌红蛋白的交叉反应性，指出某些抗体可能干扰法医或食品检测中的人源/动物源样本区分，建议优化抗体筛选流程。
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如需具体文献，建议通过PubMed或Web of Science检索关键词"Myoglobin antibody"+"diagnostic"/"assay"获取最新研究。
Myoglobin antibodies are immunological tools primarily used to detect and quantify myoglobin, a small oxygen-binding protein found in cardiac and skeletal muscle tissues. Myoglobin, consisting of 153 amino acids, facilitates oxygen storage and transport within muscle cells. Its release into the bloodstream during muscle injury—such as myocardial infarction or rhabdomyolysis—makes it a critical biomarker for diagnosing acute muscle damage.
Antibodies targeting myoglobin are typically produced by immunizing animals (e.g., rabbits, mice) with purified myoglobin, generating polyclonal or monoclonal variants. Monoclonal antibodies offer high specificity by recognizing a single epitope, while polyclonal antibodies detect multiple epitopes, enhancing sensitivity. These antibodies are widely employed in immunoassays like ELISA, Western blotting, and immunohistochemistry to assess myoglobin levels in clinical or research settings.
Clinically, myoglobin antibodies aid in early detection of heart attacks, as myoglobin rises within 1–3 hours post-injury. They also help differentiate muscle-specific conditions from other pathologies. However, cross-reactivity with similar proteins (e.g., hemoglobin) may occur, necessitating rigorous validation. In research, these antibodies contribute to studies on muscle physiology, ischemia-reperfusion injury, and muscular dystrophies. Advances in recombinant antibody technology continue to refine their specificity and diagnostic accuracy, reinforcing their role in both medical and experimental contexts.
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