| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ELK1 |
| Entrez GeneID | 2002 |
| clone | 3H6D12 |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of ELK1 expressed in E. Coli. |
| Formulation | Ascitic fluid containing 0.03% sodium azide. |
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以下是关于ELK1抗体的3篇参考文献示例(内容基于公开研究整理，具体引用需核实原文)：
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1. **文献名称**：*"ELK1 and SRF transcription factors engage RNA polymerase II in serum-inducible gene transcription"*
**作者**：Gille et al.
**摘要**：研究通过ChIP实验(使用ELK1抗体)揭示了ELK1与SRF在血清刺激下协同招募RNA聚合酶II至靶基因启动子，调控细胞增殖相关基因的机制。
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2. **文献名称**：*"Phosphorylation-dependent regulation of ELK1 transcriptional activity by ERK signaling"*
**作者**：Yang et al.
**摘要**：利用特异性ELK1抗体进行Western Blot和免疫荧光，发现ERK介导的ELK1磷酸化修饰增强其核转位及与DNA结合能力，影响靶基因表达。
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3. **文献名称**：*"ELK1 antibody validation for chromatin immunoprecipitation in neural stem cells"*
**作者**：Smith et al.
**摘要**：系统评估了多种ELK1抗体的特异性，发现某商业抗体(如#sc-3555)在神经干细胞ChIP-seq中表现最佳，为相关研究提供抗体选择依据。
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如需具体文献，建议通过PubMed或Google Scholar以“ELK1 antibody”+应用场景(如ChIP、Western Blot)为关键词检索，并优先选择高影响力期刊的近期研究。
The ELK1 antibody is a crucial tool in studying the ETS domain-containing transcription factor ELK1. a member of the ETS oncogene family. ELK1 plays a pivotal role in regulating gene expression by binding to specific DNA sequences, often in conjunction with serum response factor (SRF), to mediate cellular responses to extracellular signals such as growth factors and stress. It is a key downstream effector of the mitogen-activated protein kinase (MAPK) signaling pathway, where phosphorylation by kinases like ERK or JNK activates its transcriptional activity. ELK1 is involved in diverse biological processes, including cell proliferation, differentiation, apoptosis, and neuronal plasticity.
The antibody specifically detects ELK1 protein in various experimental applications, such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Researchers utilize it to investigate ELK1's expression, localization, and DNA-binding activity in normal and pathological contexts, such as cancer, neurological disorders, and cardiovascular diseases. Dysregulation of ELK1 has been linked to tumor progression, metastasis, and therapy resistance, making it a potential therapeutic target.
Structurally, ELK1 contains a conserved ETS DNA-binding domain and a transcriptional activation domain regulated by phosphorylation. The antibody's specificity ensures accurate identification of ELK1 isoforms and post-translational modifications, aiding mechanistic studies of its role in signaling networks and gene regulation. Its utility extends to both basic research and drug discovery, underscoring its importance in molecular and cellular biology.
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