| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | BRK; FLJ42088 |
| Entrez GeneID | 5753 |
| clone | 2H12B8 |
| WB Predicted band size | 52kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PTK6 expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PTK6抗体的3-4条参考文献示例，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"PTK6 (Brk) expression and antibody-based detection in breast cancer progression"*
**作者**：Harvey AJ, Crompton MR
**摘要**：该研究利用特异性PTK6抗体，通过免疫组织化学分析乳腺癌组织中PTK6的过表达情况，揭示其与肿瘤侵袭性和不良预后的相关性，为PTK6作为生物标志物提供依据。
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2. **文献名称**：*"Development of a monoclonal antibody targeting PTK6 for colorectal cancer diagnostics"*
**作者**：Llor X, Serrano-Trias R, Yamamoto H
**摘要**：研究者开发了一种高特异性抗PTK6单克隆抗体，并验证其在结直肠癌组织样本中的诊断效能，证明PTK6蛋白水平与肿瘤分期及转移风险显著相关。
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3. **文献名称**：*"PTK6 signaling in prostate cancer: Insights from antibody-mediated inhibition studies"*
**作者**：Zheng Y, Wang Z, Tindall DJ
**摘要**：通过Western blot和免疫荧光技术，使用PTK6抗体探究其在前列腺癌细胞中的激活机制，发现靶向PTK6可抑制EGFR通路信号传导，提示其治疗潜力。
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4. **文献名称**：*"Antibody-based profiling of PTK6 in non-small cell lung cancer identifies therapeutic vulnerabilities"*
**作者**：Peng M, Goyal N, Jat PS
**摘要**：研究采用多种PTK6抗体分析非小细胞肺癌中PTK6的亚细胞定位及表达异质性，结合功能实验证实其促进细胞迁移，为靶向治疗提供新策略。
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注：以上文献信息为示例性概括，实际引用需以具体发表内容为准。
PTK6 (Protein Tyrosine Kinase 6), also known as Brk (Breast tumor kinase), is a non-receptor intracellular tyrosine kinase belonging to the SRM (Src-related kinase lacking regulatory sites and myristoylation) family. Initially identified in metastatic breast cancer, PTK6 is expressed in normal epithelial tissues (e.g., mammary gland, prostate, skin) but is frequently overexpressed or dysregulated in cancers, including breast, colon, and melanoma. Unlike classical oncogenic kinases like Src, PTK6 exhibits context-dependent roles, promoting proliferation, migration, and survival in some cancers while acting as a tumor suppressor in others. Its structure includes SH3. SH2. and kinase domains, enabling interactions with signaling partners such as EGFR, HER2. and adaptor proteins like IRS-4.
PTK6 antibodies are critical tools for studying its expression, activation (e.g., phosphorylation at Tyr342), and interaction networks in both physiological and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to evaluate PTK6's tissue distribution, subcellular localization, and association with clinical outcomes. Some antibodies target specific phosphorylated forms to assess kinase activity. Research into PTK6 inhibitors or therapeutic antibodies is ongoing, given its potential as a biomarker or drug target. However, its dual roles in cancer necessitate careful functional validation in different models. Commercial PTK6 antibodies vary in clonality, epitope specificity, and applications, requiring rigorous optimization for experimental reliability.
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