Mouse Monoclonal MBPTag Antibody

中文名： MBP Tag抗体
别名： Mal

货号: IPD20214

Price： ￥780

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/500-1/1000	Tag
IF	咨询技术	Tag
IHC	1/100 - 1/1000	Tag
ICC	1/100 - 1/1000	Tag
FCM	1/200 - 1/400	Tag
Elisa	1/10000	Tag

产品详情

Aliases	Mal
clone	3G1A3
Host/Isotype	Mouse IgG1
Antibody Type	Tag Antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Tag
Immunogen	Recombinant fusion protein with Maltose binding protein tag.
Formulation	Ascitic fluid containing 0.03% sodium azide.

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Western blot analysis using MBP mouse mAb against various fusion protein with MBP tag.

参考文献

以下是关于MBP Tag抗体的参考文献示例(内容为模拟生成，非真实文献)：

1. **文献名称**：*Development of a High-Affinity Monoclonal Antibody Against MBP Tag for Enhanced Protein Detection*

**作者**：Smith J. et al.

**摘要**：本研究报道了一种针对MBP标签的高特异性单克隆抗体的开发，通过杂交瘤技术筛选获得。该抗体在Western blot和免疫荧光中表现出高灵敏度，适用于原核表达系统中MBP融合蛋白的检测，并验证了其与MBP标签的强结合能力，无交叉反应性。

2. **文献名称**：*Application of MBP-Specific Polyclonal Antibodies in Affinity Purification of Fusion Proteins*

**作者**：Li Y. & Zhang R.

**摘要**：文章比较了多种MBP标签多克隆抗体在亲和层析中的应用，证明其可从大肠杆菌裂解液中高效纯化MBP融合蛋白。通过优化洗脱条件，抗体偶联的树脂实现了>90%的蛋白回收率，且适用于规模化生产。

3. **文献名称**：*Evaluation of Commercial MBP-Tag Antibodies for Cross-Species Reactivity*

**作者**：Nakamura K. et al.

**摘要**：系统评估了市售MBP标签抗体在哺乳动物、酵母及昆虫细胞系统中的交叉反应性。结果显示，部分抗体在真核表达体系中存在非特异性结合，提示需根据实验体系选择抗体，并提出了验证方案。

4. **文献名称**：*A Dual-Tag System Combining MBP and His Tags for Rapid Protein Detection and Purification*

**作者**：Wang H. et al.

**摘要**：提出了一种MBP/His双标签系统，结合对应抗体的协同作用，可在ELISA和免疫沉淀中实现低背景、高信噪比的检测。MBP抗体用于初步富集，His标签用于精细纯化，显著提升重组蛋白制备效率。

(注：以上文献为示例性内容，实际引用请通过学术数据库检索核实。)

背景信息

**Background of MBP-Tag Antibodies**

MBP (Maltose-Binding Protein) is a 42 kDa protein derived from the *malE* gene of *Escherichia coli*, widely employed as a fusion partner in recombinant protein expression. The MBP-tag system was developed to enhance the solubility and proper folding of target proteins, particularly for eukaryotic proteins expressed in prokaryotic systems like *E. coli*. Its ability to bind maltose facilitates affinity-based purification via amylose resin, simplifying protein isolation.

MBP-tag antibodies are immunodetection tools specifically designed to recognize the MBP moiety in fusion proteins. These antibodies, often produced in hosts like rabbits or mice, enable researchers to detect, quantify, or localize MBP-tagged proteins in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Their high specificity arises from epitopes within the MBP sequence, ensuring minimal cross-reactivity with endogenous proteins.

The popularity of MBP-tag antibodies stems from their dual utility: they not only validate protein expression but also confirm the success of purification workflows. Compared to other tags (e.g., GST or His-tags), MBP often improves yields and solubility of challenging proteins, making its antibodies critical in structural biology and functional studies. Additionally, commercial MBP antibodies are typically well-characterized, offering reliability for standardized protocols.

In summary, MBP-tag antibodies are indispensable in recombinant protein research, bridging protein engineering needs with analytical applications, while leveraging the solubility-enhancing properties of the MBP system.