Mouse Monoclonal humanAlbumin Antibody

Human albumin antibodies are essential tools in biomedical research and clinical diagnostics, targeting human serum albumin (HSA), the most abundant plasma protein. HSA, synthesized by the liver, maintains osmotic pressure, transports hormones, fatty acids, and drugs, and serves as a biomarker for liver and kidney function. Antibodies against HSA are typically produced in immunized hosts (e.g., rabbits, mice) using purified albumin as an antigen, resulting in either polyclonal (recognizing multiple epitopes) or monoclonal (specific to a single epitope) forms. These antibodies exhibit high specificity and affinity, enabling applications such as ELISA, Western blotting, immunohistochemistry, and flow cytometry to quantify or localize albumin in biological samples. Clinically, they aid in diagnosing conditions like proteinuria or cirrhosis by detecting abnormal albumin levels. In research, they help standardize samples, remove albumin interference in proteomic studies, or study albumin's role in drug delivery. Recent advancements include recombinant antibodies engineered for enhanced stability and reduced cross-reactivity. Validation parameters, such as sensitivity, specificity, and lot consistency, are critical to ensure reproducibility. Despite albumin's high abundance, its complex structure (66.5 kDa, three homologous domains) and post-translational modifications necessitate rigorous antibody validation to avoid cross-reactivity with related proteins.