Mouse Monoclonal TBX5 Antibody

The TBX5 antibody is a crucial tool in biomedical research for studying the TBX5 protein, a member of the T-box transcription factor family. TBX5 plays a pivotal role in embryonic development, particularly in cardiac and limb formation. Mutations in the TBX5 gene are linked to Holt-Oram syndrome, a genetic disorder characterized by congenital heart defects and upper limb abnormalities. Researchers use TBX5 antibodies to detect, localize, and quantify TBX5 expression in tissues and cells, enabling investigations into its regulatory mechanisms and interactions with signaling pathways like BMP and FGF. These antibodies are widely applied in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP). Monoclonal and polyclonal variants offer specificity and versatility across experimental models. Beyond basic research, TBX5 antibodies hold diagnostic potential for identifying TBX5-related pathologies and evaluating gene therapy outcomes. However, variability in antibody performance (e.g., cross-reactivity, batch differences) necessitates rigorous validation. Ongoing advancements in antibody engineering aim to enhance their precision, supporting deeper insights into developmental biology and congenital disease mechanisms.