| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CHMP7 |
| Uniprot No | Q8WUX9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-453aa |
| 氨基酸序列 | MWSPEREAEAPAGGDPAGLLPPEWEEDEERMSFLFSAFKRSREVNSTDWDSKMGFWAPLVLSHSRRQGVVRLRLRDLQEAFQRKGSVPLGLATVLQDLLRRGELQRESDFMASVDSSWISWGVGVFLLKPLKWTLSNMLGDNKVPAEEVLVAVELLKEKAEEVYRLYQNSPLSSHPVVALSELSTLCANSCPDERTFYLVLLQLQKEKRVTVLEQNGEKIVKFARGPRAKVSPVNDVDVGVYQLMQSEQLLSRKVESLSQEAERCKEEARRACRAGKKQLALRSLKAKQRTEKRIEALHAKLDTVQGILDRIYASQTDQMVFNAYQAGVGALKLSMKDVTVEKAESLVDQIQELCDTQDEVSQTLAGGVTNGLDFDSEELEKELDILLQDTTKEPLDLPDNPRNRHFTNSVPNPRISDAELEAELEKLSLSEGGLVPSSKSPKRQLEPTLKPL |
| 预测分子量 | 56.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于CHMP7重组蛋白的模拟参考文献示例(注：部分内容为基于真实研究方向模拟的文献概括，非真实存在)：
1. **《CHMP7 mediates ESCRT-III complex function in nuclear envelope reformation》**
- 作者：Smith A et al.
- 摘要：研究发现CHMP7重组蛋白在体外实验中通过与ESCRT-III复合体成员相互作用，调控核膜重构过程，揭示了其在细胞分裂后期核膜完整性修复中的关键作用。
2. **《Structural analysis of recombinant CHMP7 reveals its role in liquid-liquid phase separation》**
- 作者：Li X et al.
- 摘要：通过体外表达纯化CHMP7重组蛋白并结合冷冻电镜技术，揭示了其寡聚化结构特征及在核周膜区相分离中的功能，为神经退行性疾病相关的核膜异常提供机制解释。
3. **《CHMP7 dysfunction induces cytoplasmic TDP-43 aggregation in ALS models》**
- 作者：Johnson R et al.
- 摘要：利用重组CHMP7蛋白进行功能回补实验，证明其缺失会导致TDP-43蛋白异常聚集，提示CHMP7在肌萎缩侧索硬化症(ALS)病理中的潜在调控作用。
(注：实际文献需通过PubMed/Google Scholar等平台以关键词"CHMP7 recombinant"检索近年研究。)
CHMP7 (Charged Multivesicular Body Protein 7) is a key component of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery, which mediates critical membrane remodeling processes in eukaryotic cells. As part of the ESCRT-III subcomplex, CHMP7 plays a specialized role in nuclear envelope reformation during mitotic exit and in repairing nuclear membrane ruptures. Unlike other ESCRT-III proteins, CHMP7 contains a unique N-terminal disordered region and a conserved C-terminal domain with positively charged residues, enabling it to sense membrane curvature and recruit downstream ESCRT-III subunits.
Recombinant CHMP7 protein is produced using heterologous expression systems (e.g., E. coli or mammalian cells) for structural and functional studies. Its purification often involves affinity chromatography tags (e.g., His-tag) followed by biochemical characterization. Research using recombinant CHMP7 has revealed its ability to oligomerize into spiral filaments on membranes, interact with binding partners like LEM2/BAF, and coordinate with the AAA+ ATPase VPS4 for membrane scission. Dysregulation of CHMP7 is linked to pathological conditions, including cancer metastasis, neurodegeneration, and defective autophagy.
Current studies focus on its role in maintaining nuclear integrity, viral budding inhibition, and crosstalk with other ESCRT components. Recombinant protein tools enable in vitro reconstitution of ESCRT-mediated processes and screening for modulators targeting nuclear envelope repair pathways.
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