| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PATL1 |
| Uniprot No | Q86TB9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1–770aa |
| 氨基酸序列 | MFRYESLEDCPLDEDEDAFQGLGEEDEEIDQFNDDTFGSGAVDDDWQEAHERLAELEEKLPVAVNEQTGNGERDEMDLLGDHEENLAERLSKMVIENELEDPAIMRAVQTRPVLQPQPGSLNSSIWDGSEVLRRIRGPLLAQEMPTVSVLEYALPQRPPQGPEDDRDLSERALPRRSTSPIIGSPPVRAVPIGTPPKQMAVPSFTQQILCPKPVHVRPPMPPRYPAPYGERMSPNQLCSVPNSSLLGHPFPPSVPPVLSPLQRAQLLGGAQLQPGRMSPSQFARVPGFVGSPLAAMNPKLLQGRVGQMLPPAPGFRAFFSAPPSATPPPQQHPPGPGPHLQNLRSQAPMFRPDTTHLHPQHRRLLHQRQQQNRSQHRNLNGAGDRGSHRSSHQDHLRKDPYANLMLQREKDWVSKIQMMQLQSTDPYLDDFYYQNYFEKLEKLSAAEEIQGDGPKKERTKLITPQVAKLEHAYKPVQFEGSLGKLTVSSVNNPRKMIDAVVTSRSEDDETKEKQVRDKRRKTLVIIEKTYSLLLDVEDYERRYLLSLEEERPALMDDRKHKICSMYDNLRGKLPGQERPSDDHFVQIMCIRKGKRMVARILPFLSTEQAADILMTTARNLPFLIKKDAQDEVLPCLLSPFSLLLYHLPSVSITSLLRQLMNLPQSAATPALSNPHLTAVLQNKFGLSLLLILLSRGEDLQSSDPATESTQNNQWTEVMFMATRELLRIPQAALAKPISIPTNLVSLFSRYVDRQKLNLLETKLQLVQGIR |
| 预测分子量 | 89.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于PATL1重组蛋白的参考文献示例(非真实文献，仅供格式参考)：
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1. **文献名称**: *"Recombinant expression and functional characterization of human PATL1 in mRNA decay regulation"*
**作者**: Smith J, et al.
**摘要**: 本研究报道了人源PATL1蛋白在大肠杆菌系统中的重组表达与纯化，通过体外RNA结合实验证实PATL1通过N端结构域特异性识别含AU-rich元件的mRNA，并参与调控靶标mRNA的稳定性。
2. **文献名称**: *"Structural insights into PATL1 oligomerization and its role in cytoplasmic granule assembly"*
**作者**: Lee S, et al.
**摘要**: 利用昆虫细胞表达系统获得重组PATL1蛋白，结合冷冻电镜技术解析其三维结构，揭示PATL1通过C端卷曲螺旋结构域形成二聚体，对应激颗粒的形成具有关键作用。
3. **文献名称**: *"PATL1 interacts with decapping enzymes: Biochemical analysis using recombinant proteins"*
**作者**: Zhang Y, et al.
**摘要**: 通过共表达纯化PATL1与DCP1/DCP2复合体，证明重组PATL1直接结合去腺苷酸化酶复合物，增强其mRNA脱帽活性，为PATL1在转录后调控中的功能提供机制解释。
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**建议**：如需真实文献，请通过PubMed、Google Scholar或Web of Science检索关键词"PATL1 recombinant" "PATL1 protein purification"等，并筛选近年发表的实验性研究。
PATL1 (Patatin-like phospholipase domain-containing protein 1) is a member of the patatin-like phospholipase family, characterized by a conserved catalytic dyad (serine-aspartate) within its patatin domain. This enzyme hydrolyzes phospholipids, playing roles in lipid metabolism, membrane remodeling, and cell signaling. Human PATL1. encoded by the PNPLA8 gene, is ubiquitously expressed but shows elevated activity in tissues with high energy demands, such as skeletal muscle and the liver.
Research links PATL1 to cellular stress responses, including endoplasmic reticulum (ER) homeostasis and autophagy. It regulates lipid droplet dynamics by modulating triglyceride hydrolysis, influencing energy storage and release. Dysregulation of PATL1 has been implicated in metabolic disorders, including non-alcoholic fatty liver disease (NAFLD) and insulin resistance. Recent studies also suggest its involvement in cancer progression, potentially through lipid signaling pathways affecting cell proliferation and apoptosis.
Recombinant PATL1 protein is produced using expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties and interactions. Purified variants enable in vitro assays to characterize enzymatic kinetics, substrate specificity, and inhibitor screening. Structural studies using recombinant PATL1 have revealed conformational changes during lipid binding, providing insights into its mechanism of action.
Current applications include drug discovery targeting metabolic diseases and mechanistic studies of lipid-associated pathologies. Challenges remain in understanding tissue-specific regulation and post-translational modifications impacting its activity. Ongoing research aims to clarify PATL1's physiological roles and therapeutic potential in metabolic syndrome and cancer.
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