| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERVK-5 |
| Uniprot No | Q9HDB9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-667aa |
| 氨基酸序列 | GQTKSKTKSKYASYLSFIKILLKRGGVRVSTKNLIKLFQIIEQFCPWFPEQGTLDLKDWKRIGEELKQAGRKGNIIPLTVWNDWAIIKAALEPFQTKEDSVSVSDAPGSCVIDCNEKTGRKSQKETESLHCEYVTEPVMAQSTQNVDYNQLQGVIYPETLKLEGKGPELVGPSESKPRGPSPLPAGQVPVTLQPQTQVKENKTQPPVAYQYWPPAELQYLPPPESQYGYPGMPPALQGRAPYPQPPTVRLNPTASRSGQGGTLHAVIDEARKQGDLEAWRFLVILQLVQAGEETQVGAPARAETRCEPFTMKMLKDIKEGVKQYGSNSPYIRTLLDSIAHGNRLTPYDWESLAKSSLSSSQYLQFKTWWIDGVQEQVRKNQATKPTVNIDADQLLGTGPNWSTINQQSVMQNEAIEQVRAICLRAWGKIQDPGTAFPINSIRQGSKEPYPDFVARLQDAAQKSITDDNARKVIVELMAYENANPECQSAIKPLKGKVPAGVDVITEYVKACDGIGGAMHKAMLMAQAMRGLTLGGQVRTFGKKCYNCGQIGHLKRSCPVLNKQNIINQAITAKNKKPSGLCPKCGKGKHWANQCHSKFDKDGQPLSGNRKRGQPQAPQQTGAFPVQLFVPQGFQGQQPLQKIPPLQGVSQLQQSNSCPAPQQAAPQ |
| 预测分子量 | 80.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ERVK-5重组蛋白的参考文献示例(注：部分内容基于真实研究背景调整，但非真实文献，仅供参考)：
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1. **文献名称**：*Expression and Purification of Recombinant ERVK-5 Envelope Protein in Mammalian Cells*
**作者**：Smith J, et al.
**摘要**：本研究描述了在HEK293细胞中高效表达并纯化ERVK-5包膜糖蛋白的方法，验证了其抗原性，为后续免疫学研究提供工具。
2. **文献名称**：*Structural Characterization of ERVK-5 Recombinant Protein Reveals Functional Domains*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了ERVK-5重组蛋白的3D结构，揭示了其与宿主细胞相互作用的潜在区域，支持其在病毒感染机制中的角色假说。
3. **文献名称**：*ERVK-5 Recombinant Protein Induces Pro-inflammatory Cytokines in Human Macrophages*
**作者**：García R, et al.
**摘要**：实验表明，ERVK-5重组蛋白可激活巨噬细胞NF-κB通路，促发炎症因子分泌，提示其可能与自身免疫疾病相关。
4. **文献名称**：*Development of an ELISA Assay Using Recombinant ERVK-5 for Serological Screening*
**作者**：Wang Y, et al.
**摘要**：利用重组ERVK-5蛋白建立高灵敏ELISA检测法，用于人群血清中ERVK抗体的筛查，证实其在某些癌症患者中抗体滴度显著升高。
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**注意**：以上文献为示例，实际研究中建议通过PubMed或Google Scholar以关键词“ERVK-5 recombinant protein”“HERV-K envelope”等检索最新成果。若需具体文献，可提供进一步研究方向以便协助筛选。
ERVK-5 is a recombinant protein derived from the envelope (Env) glycoprotein of human endogenous retrovirus type K (HERV-K), specifically the HML-2 subgroup. HERVs are remnants of ancient retroviral infections integrated into the human genome, constituting ~8% of human DNA. Among HERV families, HERV-K (HML-2) is evolutionarily the youngest and retains open reading frames encoding functional proteins, including Env. The Env protein typically consists of surface (SU) and transmembrane (TM) subunits, mediating viral entry through receptor binding and membrane fusion. ERVK-5 is engineered to express key domains of the HERV-K Env, particularly the receptor-binding region of SU, which may interact with host cell receptors like ASCT1/2.
Research on ERVK-5 focuses on its potential roles in human physiology and disease. HERV-K Env proteins are implicated in placental development, immune modulation, and cellular differentiation. However, aberrant expression of HERV-K Env is linked to pathologies, including cancers (e.g., melanoma, breast cancer), neurodegenerative diseases (e.g., ALS), and autoimmune disorders. ERVK-5 serves as a tool to study these associations, enabling investigation of Env-mediated signaling pathways, immune responses, and pathogenic mechanisms. For example, recombinant ERVK-5 is used to assess its immunogenicity, potential for triggering pro-inflammatory responses, or interference with endogenous retroviral silencing.
Structurally, ERVK-5 is produced via mammalian expression systems to ensure proper glycosylation and folding. Its applications include antibody generation, receptor interaction studies, and therapeutic target validation. Recent studies also explore ERVK-5 in biomarker development, as HERV-K Env proteins are detectable in patient sera. Despite its utility, challenges remain in understanding its precise biological roles, given HERV-K’s genetic variability and context-dependent expression. ERVK-5 thus represents a critical reagent for unraveling HERV-K’s contributions to health and disease.
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