| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | glk |
| Uniprot No | Q8IVH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 氨基酸序列 | MNPGFDLSRRNPQEDFELIQRIGSGTYGDVYKARNVNTGELAAIKVIKLE PGEDFAVVQQEIIMMKDCKHPNIVAYFGSYLRRDKLWICMEFCGGGSLQD IYHVTGPLSELQIAYVSRETLQGLYYLHSKGKMHRDIKGANILLTDNGHV KLADFGVSAQITATIAKRKSFIGTPYWMAPEVAAVERKGGYNQLCDLWAV GITAIELAELQPPMFDLHPMRALFLMTKSNFQPPKLKDKMKWSNSFHHFV KMALTKNPKKRPTAEKLLQHPFVTQHLTRSLAIELLDKVNNPDHSTYHDF DDDDPEPLVAVPHRIHSTSRNVREEKTRSEITFGQVKFDPPLRKETEPHH ELPDSDGFLD SSEEIYYTARSNLDLQLEYG |
| 预测分子量 | 70 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GLK(Glucokinase/GLK转录因子)重组蛋白的3篇代表性文献示例(内容为模拟生成，非真实文献)：
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1. **文献名称**: "Recombinant Human GLK Protein Expression and Its Role in Hepatic Glucose Sensing"
**作者**: Zhang, L., et al.
**摘要**: 研究通过大肠杆菌系统表达重组人源GLK蛋白，优化纯化条件后验证其酶学活性，并探讨其在肝细胞葡萄糖代谢调控中的作用，为糖尿病药物靶点研究提供基础。
2. **文献名称**: "Structural Analysis of Arabidopsis GLK Transcription Factor by Recombinant Protein Crystallography"
**作者**: Wang, Y., et al.
**摘要**: 利用昆虫表达系统获得拟南芥GLK重组蛋白，通过X射线晶体学解析其三维结构，揭示其DNA结合域的关键氨基酸残基，阐明其在叶绿体发育中的分子机制。
3. **文献名称**: "GLK Recombinant Protein Enhances Antitumor Immunity via MAPK Pathway Modulation"
**作者**: Chen, H., et al.
**摘要**: 构建哺乳动物细胞表达的GLK重组蛋白，实验证明其通过调控MAPK信号通路抑制肿瘤细胞增殖，并在小鼠模型中验证其免疫增强效应，提示其潜在抗癌应用价值。
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如需真实文献，建议通过PubMed或Web of Science检索关键词“GLK recombinant protein”或“Glucokinase recombinant”。
**Background of GLK Recombinant Protein**
GLK (Germinal Center Kinase-like kinase), also known as MAP4K3. is a serine/threonine kinase belonging to the mammalian Ste20-like kinase family. It plays a critical role in regulating cellular stress responses, immune signaling, and metabolic pathways. Structurally, GLK contains a conserved N-terminal kinase domain and a C-terminal regulatory region, enabling interactions with downstream effectors. Research highlights its involvement in mTORC1 signaling, amino acid sensing, and T-cell activation, linking it to processes like cell growth, autophagy, and immune regulation.
The production of GLK recombinant protein leverages recombinant DNA technology, where the GLK gene is cloned into expression vectors (e.g., bacterial or mammalian systems like *E. coli* or HEK293 cells) to enable large-scale protein synthesis. Affinity chromatography (e.g., His-tag purification) ensures high purity, followed by functional validation via kinase assays or Western blotting.
GLK has garnered attention in disease research, particularly cancer and autoimmune disorders. Overexpression of GLK correlates with tumor progression and therapy resistance, while its role in T-cell hyperactivation implicates it in conditions like lupus. Recombinant GLK protein facilitates *in vitro* studies to dissect its signaling mechanisms, screen kinase inhibitors, and develop targeted therapies. Additionally, it serves as an antigen for antibody production in diagnostic tools.
Overall, GLK recombinant protein is a vital tool for unraveling its biological functions and translational potential, bridging molecular insights to therapeutic innovation.
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