| 纯度 | >95%SDS-PAGE. |
| 种属 | Escherichia coli |
| 靶点 | glsA1 |
| Uniprot No | P77454 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-310aa |
| 氨基酸序列 | MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVAIVTCDG NVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNS VIALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQ VALSDEVNQSEQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLL NTIELATLGATLAAGGVNPLTHKRVLQADNVPYILAEMMMEGLYGRSGDW AYRVGLPGKSGVGGGILAVVPGVMGIAAFSPPLDEDGNSVRGQKMVASVA KQLGYNVFKG |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于 **glsA1重组蛋白** 的示例参考文献(虚构内容，仅供格式参考)：
---
1. **标题**: *Heterologous Expression and Functional Characterization of Recombinant glsA1 in Escherichia coli*
**作者**: Zhang, L.; Wang, H. et al.
**摘要**: 本研究成功在大肠杆菌中表达了glsA1重组蛋白，通过亲和层析纯化并验证其酶活性。结果表明，重组glsA1具有显著的谷氨酰胺水解能力，为其在代谢疾病治疗中的应用提供了依据。
2. **标题**: *Structural Insights into glsA1: A Novel Antibiotic Resistance Factor*
**作者**: Patel, R.; Kumar, S. et al.
**摘要**: 通过X射线晶体学解析glsA1重组蛋白的三维结构，发现其独特的底物结合域可能参与细菌抗生素耐药性机制，为靶向药物设计提供了结构基础。
3. **标题**: *Optimization of glsA1 Recombinant Protein Production in Pichia pastoris*
**作者**: García, M.; Silva, J. et al.
**摘要**: 在毕赤酵母系统中优化glsA1重组蛋白的表达条件，获得高产量活性蛋白，并验证其在工业酶催化中的潜在应用价值。
4. **标题**: *Role of glsA1 in Pathogen Metabolism: A Recombinant Protein Approach*
**作者**: Chen, X.; Li, Y. et al.
**摘要**: 利用重组glsA1蛋白研究其在病原菌代谢通路中的作用，发现敲除glsA1会显著抑制细菌生长，提示其作为抗菌靶点的潜力。
---
**注意**：以上文献为示例，实际研究中请通过 **PubMed/Google Scholar** 等平台检索真实文献(关键词如“glsA1 recombinant protein”或“glsA1 gene function”)。若需具体文献协助，请提供更多蛋白背景信息(如物种、功能等)。
**Background of GLS1 Recombinant Protein**
Glutaminase 1 (GLS1), encoded by the *GLS* gene, is a mitochondrial enzyme critical for cellular metabolism, catalyzing the hydrolysis of glutamine to glutamate. This reaction fuels the tricarboxylic acid (TCA) cycle, supporting energy production and biosynthesis in rapidly proliferating cells, including cancer cells. Two major isoforms exist: kidney-type glutaminase (KGA) and glutaminase C (GAC), generated via alternative splicing. GAC, a shorter isoform, exhibits heightened enzymatic activity and is frequently overexpressed in tumors, linking it to cancer progression and therapeutic resistance.
Recombinant GLS1 proteins, such as GLS1A (a common designation for engineered variants), are produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional and structural studies. These proteins retain enzymatic activity, enabling research into glutamine metabolism's role in diseases and drug discovery. For instance, GLS1 inhibitors like CB-839 (in clinical trials) target glutamine addiction in cancers, underscoring the protein's biomedical relevance.
The production of recombinant GLS1 involves cloning the *GLS* gene into expression vectors, followed by purification via affinity tags (e.g., His-tag). Structural analyses (e.g., X-ray crystallography) using recombinant GLS1 have revealed mechanisms of catalysis and regulation, aiding inhibitor design. Additionally, recombinant proteins facilitate *in vitro* assays to screen small molecules or study post-translational modifications impacting enzyme activity.
Overall, GLS1 recombinant proteins serve as vital tools for dissecting metabolic reprogramming in diseases and advancing targeted therapies, particularly in oncology. Their applications span basic research, drug development, and biomarker studies, highlighting their importance in precision medicine.
×
