| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNAI2 |
| Uniprot No | P04899-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-339aa |
| 氨基酸序列 | MEYAGHLPASSAQGTILACTSCTGAGESGKSTIVKQMKIIHEDGYSEEEC RQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPSRADDARQLFALSCTAEE QGVLPDDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQS DYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCF EGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSII LFLNKKDLFEEKITHSPLTICFPEYTGANKYDEAASYIQSKFEDLNKRKD TKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF |
| 预测分子量 | 63 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNAI2重组蛋白的参考文献示例(内容为虚构示例，仅作格式参考)：
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1. **文献名称**: "Expression and functional characterization of recombinant GNAI2 in mammalian cells"
**作者**: Zhang L, et al.
**摘要**: 研究通过哺乳动物表达系统成功表达并纯化GNAI2重组蛋白，验证其在G蛋白偶联受体(GPCR)信号通路中的抑制活性，证明其可有效调节cAMP水平。
2. **文献名称**: "Structural insights into GNAI2 activation and GTPase activity"
**作者**: Thompson R, et al.
**摘要**: 利用X射线晶体学解析GNAI2重组蛋白的三维结构，揭示其与GTP结合及水解的关键结构域，为靶向GNAI2的分子机制研究提供基础。
3. **文献名称**: "Role of recombinant GNAI2 in cancer cell migration assays"
**作者**: Singh M, et al.
**摘要**: 通过体外实验证明，重组GNAI2蛋白过表达可显著抑制乳腺癌细胞的迁移能力，提示其在肿瘤转移中的潜在调控作用。
4. **文献名称**: "Optimization of prokaryotic expression system for high-yield GNAI2 production"
**作者**: Chen H, et al.
**摘要**: 改进大肠杆菌表达系统，实现GNAI2重组蛋白的高效可溶性表达，并通过亲和层析纯化获得高纯度蛋白，适用于大规模生化研究。
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注：以上文献为模拟示例，实际引用需以真实发表文献为准。建议通过PubMed、Web of Science等平台检索关键词“GNAI2 recombinant protein”获取最新研究。
**Background of GNAI2 Recombinant Protein**
GNAI2 (Guanine Nucleotide-Binding Protein Alpha Inhibiting Activity Polypeptide 2) is a member of the G protein α-subunit family, which plays a central role in intracellular signal transduction mediated by G protein-coupled receptors (GPCRs). As part of the inhibitory Gi/o subfamily, GNAI2 modulates downstream signaling by inhibiting adenylate cyclase activity, reducing intracellular cAMP levels, and regulating ion channels and kinase pathways such as MAPK. It is ubiquitously expressed and involved in diverse physiological processes, including cell proliferation, differentiation, and immune response regulation.
Recombinant GNAI2 protein is engineered through molecular cloning techniques, often expressed in *E. coli* or mammalian systems, to ensure high purity and functional activity. This engineered protein retains the GTPase activity and structural domains necessary for interacting with GPCRs, effectors, and regulators like RGS proteins. Its production enables detailed studies of G protein signaling mechanisms, including ligand-receptor interactions, conformational changes, and post-translational modifications.
Research applications of GNAI2 recombinant protein span *in vitro* assays, structural biology (e.g., X-ray crystallography), and drug discovery targeting GPCR-related pathologies. Mutations or dysregulation of GNAI2 have been linked to cancers, inflammatory diseases, and neurological disorders, making it a biomarker and therapeutic candidate. Additionally, it serves as a critical tool for elucidating signaling crosstalk and developing inhibitors or activators of G protein pathways.
In summary, GNAI2 recombinant protein provides a versatile platform for advancing both basic research and translational studies in cellular signaling and disease mechanisms.
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