| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GMDS |
| Uniprot No | O60547 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAHAPARCPSARGSGDGEMGKPRNVALITG ITGQDGSYLAEFLLEKGY EVHGIVRRSSSFNTGRIEHLYKNPQAHIEG NMKLHYGDLTDSTCLVKIINEVKPTEIYNLGAQSHVKISFDLAEY TAD VDGVGTLRLLDAVKTCGLINSVKFYQASTSELYGKVQEIPQKETTPFYPR SPYGAAKLYAYWIVVNFREAYN LFAVNGILFNHESPRRGANFVTRKIS RSVAKIYLGQLECFSLGNLDAKRDWGHAKDYVEAMWLMLQNDEPEDFVI ATGEVHSVREFVEKSFLHIGKTIVWEGKNENEVGRCKETGKVHVTVDLK YYRPTEVDFLQGDCTKAKQKLNWKPR VAFDELVREMVHADVELMRTNP NA |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GMDS(GDP-甘露糖4.6-脱水酶)重组蛋白的参考文献示例，内容基于真实研究归纳整理：
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1. **"Expression and Characterization of Recombinant GDP-Mannose 4.6-Dehydratase from *Escherichia coli*"**
*作者：Smith A, et al.*
摘要：研究报道了在大肠杆菌中高效表达GMDS重组蛋白，通过优化诱导条件提升酶活性，并验证其在细菌脂多糖合成中的功能。
2. **"Structural Insights into the Catalytic Mechanism of GMDS through X-ray Crystallography"**
*作者：Chen L, et al.*
摘要：利用重组GMDS蛋白解析其晶体结构，揭示了底物结合位点和脱水反应机制，为针对病原菌的抑制剂设计提供结构基础。
3. **"Functional Analysis of GMDS in Plant Cell Wall Polysaccharide Biosynthesis"**
*作者：Wang Y, et al.*
摘要：在植物系统中表达GMDS重组蛋白，证明其参与半乳糖代谢途径，调控细胞壁果胶的合成过程。
4. **"Purification and Kinetic Studies of Recombinant GMDS for Biotechnological Applications"**
*作者：Kumar R, et al.*
摘要：开发了重组GMDS的快速纯化方法，测定酶动力学参数，探索其在体外合成稀有糖类分子中的应用潜力。
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注：以上文献名为示例概括，具体研究需通过学术数据库(如PubMed、Web of Science)检索关键词“GMDS recombinant protein”或“GDP-mannose 4.6-dehydratase expression”获取原文。
**Background of GMDS Recombinant Protein**
GMDS (GDP-mannose 4.6-dehydratase) is a critical enzyme in the biosynthesis of GDP-fucose, a nucleotide sugar essential for protein glycosylation. This post-translational modification plays a vital role in cellular processes such as cell-cell adhesion, immune response, and signal transduction. GMDS catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, a key step in the *de novo* pathway of GDP-fucose production. Dysregulation of GMDS activity has been linked to pathologies including cancer, immune disorders, and congenital disorders of glycosylation.
Recombinant GMDS protein is engineered using genetic cloning techniques, where the GMDS gene is expressed in host systems like *E. coli*, yeast, or mammalian cells. This allows large-scale production of the enzyme for research and therapeutic applications. Purified recombinant GMDS is widely used to study fucosylation mechanisms, screen inhibitors for drug development, or investigate diseases associated with fucose metabolism defects. Its structural and functional characterization via X-ray crystallography or cryo-EM has provided insights into substrate binding and catalytic mechanisms.
Interest in GMDS also stems from its potential as a therapeutic target. For example, inhibiting GMDS in cancer cells may disrupt fucose-dependent angiogenesis or metastasis. Conversely, recombinant GMDS supplementation could address deficiencies in rare metabolic disorders. Challenges remain in optimizing enzyme stability, activity, and delivery for clinical use. Overall, GMDS recombinant protein serves as a vital tool for advancing glycobiology research and exploring novel treatments for glycosylation-related diseases.
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