| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WIZ |
| Uniprot No | O95785 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1228-1419aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGSEFRTRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCLIKKEPPAGDLAPALAEDGPPTVAPGPVQSPLPLSPLAGRPGKPGAGPAQVPRELSLTPITGAKPSATGYLGSVAAKRPLQEDRLLPAEVKAKTYIQTELPFKAKTLHEKTSHSSTEACCELCGLYFENRKALASHARAH |
| 预测分子量 | 28.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条与WIZ重组蛋白相关的模拟参考文献(注：作者及文献名为假设性示例，建议通过学术数据库核实真实文献)：
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1. **《重组WIZ蛋白在大肠杆菌中的高效表达与纯化》**
作者：Zhang Y. et al.
摘要：本研究成功构建了WIZ蛋白的重组表达载体，利用大肠杆菌表达系统实现高效可溶性表达，并通过亲和层析技术获得高纯度WIZ重组蛋白，为后续功能研究提供材料基础。
2. **《WIZ蛋白与G9a组蛋白甲基转移酶复合物的相互作用机制》**
作者：Smith L. et al.
摘要：通过体外重组蛋白互作实验，证实WIZ蛋白通过其锌指结构域与G9a酶形成稳定复合物，协同调控H3K9甲基化修饰，揭示了WIZ在表观遗传沉默中的分子机制。
3. **《WIZ重组蛋白在小鼠胚胎干细胞分化中的功能研究》**
作者：Chen R. et al.
摘要：利用重组WIZ蛋白干扰实验发现，WIZ通过抑制特定多能性基因(如Oct4)的染色质开放状态，促进胚胎干细胞向中胚层分化，表明其在细胞命运决定中的关键作用。
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**建议**：实际文献可通过PubMed或Google Scholar搜索关键词 "WIZ recombinant protein" "G9a-WIZ complex" "chromatin remodeling WIZ" 获取。
WIZ (Widely Interspaced Zinc Finger Protein) is a chromatin-associated protein encoded by the *WIZ* gene in humans, first identified in studies exploring transcriptional regulators interacting with G9a/EHMT2. a histone methyltransferase involved in epigenetic gene silencing. Structurally, WIZ contains multiple C2H2-type zinc finger domains, enabling its ability to bind DNA and recruit protein complexes to specific genomic loci. It plays a critical role in mediating chromatin remodeling and transcriptional repression through its interaction with G9a, facilitating the establishment of repressive histone marks like H3K9me2. which are essential for heterochromatin formation and gene silencing.
Functionally, WIZ is implicated in diverse cellular processes, including cell cycle regulation, differentiation, and genomic stability. Studies have linked WIZ to developmental pathways and diseases such as cancer, where dysregulation of its activity may contribute to aberrant epigenetic states. For example, WIZ-G9a complexes are known to suppress tumor suppressor genes in certain malignancies. Additionally, WIZ interacts with other chromatin modifiers, such as the CoREST complex, suggesting broader roles in epigenetic regulation beyond G9a-mediated pathways.
Recombinant WIZ protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), is widely used to study its biochemical interactions, DNA-binding specificity, and structural properties. Purified WIZ protein enables *in vitro* assays to map binding partners, screen inhibitors, or analyze chromatin dynamics. Its recombinant form also aids in developing therapeutic strategies targeting epigenetic disorders. Research utilizing WIZ recombinant proteins continues to uncover mechanistic insights into chromatin biology and disease pathogenesis, highlighting its importance as a tool for both basic and translational studies.
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