| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNAZ |
| Uniprot No | P19086 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-355aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGCRQSSEEKEAARRSRRIDRHLRSESQRQ RREIKLLLLGTSNSGKSTIVKQMKIIHSGGFNLEACKEYKPLIIYNAIDS LTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRR LWADPGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRD MTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIFCVELSG YDLKLYEDNQTSRMAESLRLFDSICNNNWFINTSLILFLNKKDLLAEKIR RIPLTICFPEYKGQNTYEEAAVYIQRQFEDLNRNKETKEIYSHFTCATDT SNIQFVFDAVTDVIIQNNLKYIGLC |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNAZ重组蛋白的3篇示例参考文献(注：内容为模拟虚构，实际文献需通过学术数据库查询)：
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1. **标题**: *"Cloning and Functional Characterization of Recombinant GNAZ Protein in Mammalian Cells"*
**作者**: Smith J.R., Lee T., et al.
**摘要**: 本研究成功克隆了人类GNAZ基因，并在HEK293细胞中实现了重组蛋白的高效表达。通过体外功能实验证实，重组GNAZ能特异性激活G蛋白偶联受体(GPCR)下游信号通路，为研究其生理作用提供了工具。
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2. **标题**: *"Structural Insights into GNAZ Activation Mechanism via X-ray Crystallography"*
**作者**: Chen L., Wang H., et al.
**摘要**: 利用X射线晶体学解析了GNAZ重组蛋白的活性与非活性构象结构，揭示了其与Gβγ亚基结合的分子机制，并发现ATP依赖性磷酸化对其信号传导的调控作用。
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3. **标题**: *"Role of Recombinant GNAZ in Neuronal Signal Transduction and Disease Modeling"*
**作者**: Gupta S., Martínez-Ruiz C., et al.
**摘要**: 通过构建GNAZ重组蛋白过表达模型，发现其在神经元突触传递中调控cAMP水平，并探讨了GNAZ功能异常与神经退行性疾病的相关性，为药物靶点开发提供依据。
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如需真实文献，建议在PubMed或Web of Science中检索关键词 **"GNAZ recombinant protein"** 或 **"GNAZ subunit expression"**，并筛选近年高被引研究。
Guanine nucleotide-binding protein G(z) subunit alpha (GNAZ) is a member of the Gα subunit family within heterotrimeric G proteins, which play pivotal roles in transmitting extracellular signals via G protein-coupled receptors (GPCRs). Encoded by the GNAZ gene, this protein is part of the Gi/o subfamily, sharing structural homology with other inhibitory Gα proteins like GNAI and GNAO. Unlike widely expressed Gα subunits, GNAZ exhibits tissue-specific expression, predominantly in the brain, adrenal glands, and platelets, suggesting specialized regulatory functions in neurotransmission, hormone secretion, and hemostasis.
Recombinant GNAZ proteins are engineered using expression systems such as E. coli or mammalian cells to study its biochemical properties and signaling mechanisms. These proteins retain key functional domains, including GTPase activity and receptor interaction sites, enabling researchers to dissect its role in modulating downstream effectors like adenylate cyclase, ion channels, and MAPK pathways. Notably, GNAZ uniquely couples to certain GPCRs, such as 5-HT1 receptors and adrenergic receptors, influencing cellular responses through cAMP inhibition or calcium signaling.
Interest in recombinant GNAZ stems from its implications in disease. Dysregulation of GNAZ signaling has been linked to neurological disorders, cardiovascular conditions, and cancer progression. For instance, altered GNAZ expression correlates with tumor metastasis and platelet hyperactivity. Pharmaceutical research leverages recombinant GNAZ to screen targeted therapies or investigate ligand-receptor dynamics, offering insights into drug design for precision medicine. Its distinct tissue localization and signaling crosstalk also make it a potential biomarker or therapeutic target. Overall, recombinant GNAZ serves as a critical tool for unraveling complex GPCR networks and advancing translational studies in cellular signaling pathologies.
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