| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PB1 |
| Uniprot No | P11712 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-162aa |
| 氨基酸序列 | MDSLVVLVLCLSCLLLLSLWRQSSGRGKLPPGPTPLPVIGNILQIGIKDISKSLTNLSKVYGPVFTLYFGLKPIVVLHGYEAVKEALIDLGEEFSGRGIFPLAERANRGFGIVFSNGKKWKEIRRFSLMTLRNFGMGKRSIEDRVQEEARCLVEELRKTKGG |
| 预测分子量 | 25.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PB1重组蛋白的参考文献及其摘要概括：
1. **《Structural insights into RNA synthesis by influenza virus polymerase》**
- 作者：Pflug, A., Lukarska, M., Resa-Infante, P., et al.
- 摘要：通过重组表达流感病毒PB1蛋白与PB2、PA亚基复合体，解析了其低温电镜结构，揭示了PB1在病毒RNA聚合酶活性中的核心作用，及其与病毒RNA模板结合的分子机制。
2. **《Recombinant PB1 protein of influenza A virus exhibits RNA polymerase activity in vitro》**
- 作者：Toyoda, T., Adyshev, D.M., Kobayashi, M., et al.
- 摘要：研究通过大肠杆菌系统重组表达并纯化流感病毒PB1蛋白，证明其单独具有基础RNA聚合酶活性，并阐明了其与PA亚基互作对酶活性的增强效应。
3. **《A recombinant influenza virus PB1 protein-based vaccine induces cross-protective immunity》**
- 作者：Chen, Z., Zhou, H., Jin, H.
- 摘要：利用昆虫细胞系统表达PB1重组蛋白，开发了一种新型流感疫苗。动物实验显示，该蛋白可诱导针对不同流感亚型的交叉保护性抗体和T细胞免疫应答。
4. **《Dynamic interaction network of influenza polymerase subunits revealed by cross-linking mass spectrometry》**
- 作者：Serna, M., Carrique, L., Ramakrishnan, B., et al.
- 摘要：通过重组表达流感病毒PB1、PB2、PA蛋白并重构聚合酶复合体，结合交联质谱技术，绘制了PB1与其他亚基的动态互作界面，为抗病毒药物靶点设计提供依据。
注：以上文献信息为示例性质，实际引用时建议通过PubMed或Web of Science核对原文准确性。研究多聚焦于PB1的结构功能、重组表达技术及其在病毒复制/疫苗中的应用。
**Background of PB1 Recombinant Protein**
The PB1 (Polymerase Basic Protein 1) recombinant protein is a critical component derived from influenza viruses, particularly influenza A. PB1 serves as a subunit of the viral RNA-dependent RNA polymerase complex, alongside PB2 and PA. This complex is essential for viral replication and transcription, enabling the virus to hijack host cell machinery to produce viral RNA. PB1 plays a central role in catalyzing RNA elongation and interacts with other polymerase subunits to ensure efficient genome replication. Its structural and functional conservation across influenza strains makes it a key target for antiviral research and vaccine development.
Recombinant PB1 is produced using genetic engineering techniques, where the PB1 gene is cloned into expression vectors (e.g., bacterial, insect, or mammalian systems) and expressed in host cells. Purification methods like affinity chromatography yield high-purity protein for research. This approach allows scalable production without handling live viruses, enhancing safety and accessibility.
Studying recombinant PB1 provides insights into polymerase assembly, RNA synthesis mechanisms, and host adaptation. It aids in identifying inhibitors for antiviral drugs and understanding drug resistance. Additionally, PB1 is explored in subunit vaccines, as it contains conserved epitopes that could elicit cross-reactive immune responses. Its role in interspecies transmission and pathogenicity further underscores its importance in pandemic preparedness.
Overall, PB1 recombinant protein is a vital tool for dissecting influenza virus biology, advancing therapeutic strategies, and improving public health responses to seasonal and emerging flu strains.
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