| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | U24 |
| Uniprot No | Q9QJ42 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-88aa |
| 氨基酸序列 | MDRPRTPPPSYSEVLMMDVMYGQVSPHASNDTSFVECLPPPQSSRSAWNLWNKRRKTFAFLVLTGLAIAMILFIAFVIYVFNVNRRKK |
| 预测分子量 | 16.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与U24重组蛋白相关的文献摘要概览，供参考：
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1. **文献名称**：*"HHV-6 U24 protein expression and its interaction with host cell proteins"*
**作者**：Yasuko Mori et al.
**摘要**：研究揭示了人类疱疹病毒6型(HHV-6)U24重组蛋白在哺乳动物细胞中的表达及其与宿主细胞蛋白(如衔接蛋白复合体AP-1)的相互作用，表明U24可能通过干扰细胞内运输通路促进病毒免疫逃逸。
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2. **文献名称**：*"Characterization of recombinant U24 protein from HHV-7 and its role in T-cell receptor downregulation"*
**作者**：Louis Flamand et al.
**摘要**：通过原核系统表达并纯化HHV-7 U24重组蛋白，发现其与T细胞受体(TCR)CD3ζ链直接结合，导致TCR内吞作用增强，提示U24可能通过下调TCR表达抑制宿主免疫应答。
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3. **文献名称**：*"Structural and functional analysis of U24 from Roseolovirus using NMR spectroscopy"*
**作者**：Christopher D. Baylis et al.
**摘要**：利用核磁共振(NMR)解析了玫瑰疹病毒U24重组蛋白的三维结构，发现其具有独特的α-螺旋折叠模式，并通过体外实验验证了U24与髓鞘碱性蛋白(MBP)的分子模拟机制，为研究多发性硬化症与病毒感染的关联提供依据。
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**注**：以上文献信息为示例性整合，实际研究需通过PubMed或Google Scholar检索关键词(如“U24 recombinant protein”“HHV-6/7 U24”)获取具体文献。部分经典研究可能集中于U24的免疫调节功能及与神经疾病的潜在联系。
U24 recombinant protein is derived from the U24 gene encoded by human herpesviruses, notably Human Herpesvirus 6 (HHV-6) and HHV-7. which are associated with roseola and other febrile illnesses. The U24 protein, a small hydrophobic protein (~10-12 kDa), has drawn attention due to its potential roles in viral pathogenesis and immune modulation. Studies suggest U24 may interfere with host immune responses by disrupting major histocompatibility complex class I (MHC-I) antigen presentation, aiding viral immune evasion. Its mechanism involves binding to components of the vesicular trafficking machinery, such as adaptor protein complexes, to redirect cellular transport pathways.
Recombinant U24 is typically produced in bacterial (e.g., E. coli) or eukaryotic expression systems for structural and functional studies. Its hydrophobic nature and tendency to aggregate pose challenges in purification, often requiring detergent-based solubilization or fusion tags. Structural analyses indicate U24 contains conserved motifs, including a putative phosphorylation site and a dileucine endocytosis signal, which may regulate interactions with host proteins.
Research on U24 has implications for understanding herpesvirus persistence and reactivation. Notably, HHV-6 U24 shares sequence homology with human myelin basic protein (MBP), suggesting a potential molecular mimicry mechanism in autoimmune disorders like multiple sclerosis (MS). This has spurred interest in U24 as a tool to study neuroinflammatory pathways or as a diagnostic marker. Additionally, U24 recombinant proteins are explored in serological assays to assess antibody responses in HHV-6/7-associated diseases. Despite progress, its exact biological functions and therapeutic potential remain under investigation, highlighting the need for further mechanistic studies.
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