| WB | 1/500 - 1/2000 | Human,Monkey |
| IF | 咨询技术 | Human,Monkey |
| IHC | 1/200 - 1/1000 | Human,Monkey |
| ICC | 技术咨询 | Human,Monkey |
| FCM | 咨询技术 | Human,Monkey |
| Elisa | 1/10000 | Human,Monkey |
| Aliases | AIM; DNMT; MCMT; CXXC9; HSN1E |
| Entrez GeneID | 1786 |
| clone | 7C11A2 |
| WB Predicted band size | 183.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Monkey |
| Immunogen | Purified recombinant fragment of human DNMT1 (AA: 1448-1594) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于DNMT1抗体的3篇参考文献，包含文献名称、作者及摘要内容的简要概括：
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1. **文献名称**：*DNMT1 is essential for mammary epithelial stem cell differentiation and mammary gland development*
**作者**：Lee J, et al.
**摘要**：该研究利用特异性DNMT1抗体，通过免疫荧光和Western blot技术，揭示DNMT1在小鼠乳腺干细胞分化和乳腺发育中的关键作用，证实其缺失导致表观遗传紊乱和分化异常。
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2. **文献名称**：*Aberrant expression of DNMT1 in colorectal cancer: A biomarker with prognostic significance*
**作者**：Zhang Y, et al.
**摘要**：通过免疫组化(使用抗人DNMT1单克隆抗体)分析结直肠癌组织，发现DNMT1高表达与肿瘤进展和患者生存率降低相关，提示其作为潜在预后标志物的价值。
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3. **文献名称**：*Validation of a commercial DNMT1 antibody for epigenetic studies in human tissues*
**作者**：Smith R, et al.
**摘要**：系统验证了一种市售DNMT1抗体在多种实验(ChIP-seq、免疫沉淀)中的特异性，确认其在正常与疾病状态样本中的可靠性，为表观遗传学研究提供技术参考。
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如果需要更详细的信息或扩展文献范围，可进一步检索PubMed或SciHub获取全文。
DNA methyltransferase 1 (DNMT1) is a critical enzyme responsible for maintaining DNA methylation patterns during replication by copying methylation marks onto newly synthesized DNA strands. This epigenetic regulation is essential for gene silencing, genomic stability, and X-chromosome inactivation. DNMT1 antibodies are indispensable tools in studying its role in these processes, enabling detection, localization, and functional analysis in various experimental setups.
DNMT1 dysregulation is linked to diseases such as cancer (where overexpression promotes tumorigenesis), neurodegenerative disorders (e.g., Alzheimer’s), and autoimmune conditions. Antibodies targeting DNMT1 facilitate research into its involvement in these pathologies by enabling techniques like Western blotting, immunofluorescence, chromatin immunoprecipitation (ChIP), and immunohistochemistry.
These antibodies typically recognize specific epitopes or domains (e.g., the N-terminal regulatory region or C-terminal catalytic domain), allowing researchers to investigate post-translational modifications or protein interactions. Validation across species (human, mouse, rat) and sample types (cell lysates, tissues) ensures broad applicability.
Recent studies also explore DNMT1's non-canonical roles, such as in DNA repair or cellular differentiation, further driving demand for high-specificity antibodies. However, challenges remain, including cross-reactivity with other DNMTs (e.g., DNMT3A/B) and variability in experimental conditions. Overall, DNMT1 antibodies are pivotal in advancing epigenetic research and therapeutic targeting of methylation-related diseases.
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