| 纯度 | >85%SDS-PAGE. |
| 种属 | Mouse |
| 靶点 | GOT1 |
| Uniprot No | P05201 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-413aa |
| 氨基酸序列 | APPSVFAQV PQAPPVLVFK LTADFRDDPD PRKVNLGVGA YRTDESQPWV LPVVRKVEQK IANDNSLNHE YLPILGLAEF RSCASRLVLG DNSLAIRENR VGGVQSLGGT GALRIGADFL GRWYNGTDNK NTPIYVSSPT WENHNAVFSA AGFKDIRPYC YWDAEKRGLD LQGFLNDLEN APEFSIFVLH ACAHNPTGTD PTPEQWKQIA AVMQRRFLFP FFDSAYQGFA SGDLEKDAWA IRYFVSEGFE LFCAQSFSKN FGLYNERVGN LTVVGKESDS VLRVLSQMEK IVRITWSNPP AQGARIVAAT LSDPELFKEW KGNVKTMADR ILTMRSELRA RLEALKTPGT WSHITEQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINM CGLTTKNLDY VATSIHEAVT KIQ |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and functional characterization of recombinant human glutamate oxaloacetate transaminase 1 (GOT1)"**
- **作者**: Zhang Y, et al.
- **摘要**: 该研究通过大肠杆菌表达系统成功制备重组人源GOT1蛋白，解析其晶体结构，并验证其催化天冬氨酸与α-酮戊二酸转化为草酰乙酸和谷氨酸的酶活性，为靶向GOT1的疾病治疗提供结构基础。
2. **"GOT1 regulates cellular redox homeostasis in pancreatic cancer via malate-aspartate shuttle"**
- **作者**: Son J, et al.
- **摘要**: 文章揭示重组GOT1在胰腺癌细胞中通过苹果酸-天冬氨酸穿梭维持NAD+/NADH平衡，促进肿瘤生长，抑制GOT1活性可增强氧化应激并抑制癌细胞增殖。
3. **"Kinetic analysis of recombinant GOT1 enzyme reveals its role in alanine metabolism"**
- **作者**: Brown TP, et al.
- **摘要**: 通过体外酶动力学实验，证明重组GOT1在丙氨酸代谢中的双向催化功能，并探讨其底物特异性及pH依赖性，为代谢疾病研究提供依据。
4. **"Development of a high-throughput assay for screening GOT1 inhibitors using recombinant protein"**
- **作者**: Lee SM, et al.
- **摘要**: 研究建立基于重组GOT1蛋白的高通量抑制剂筛选平台，鉴定多个小分子化合物，为开发抗肿瘤或代谢性疾病药物奠定基础。
(注：以上文献信息为示例性概括，实际引用需以真实文献为准。)
**Background of GOT1 Recombinant Protein**
Glutamic-oxaloacetic transaminase 1 (GOT1), also known as aspartate aminotransferase (AST), is a key enzyme involved in cellular amino acid metabolism and the malate-aspartate shuttle. It catalyzes the reversible transfer of an amino group between aspartate and glutamate, linking the urea cycle, tricarboxylic acid (TCA) cycle, and nucleotide biosynthesis. GOT1 is predominantly localized in the cytosol, while its mitochondrial counterpart, GOT2. functions in a similar pathway but within mitochondria. This compartmentalization allows fine-tuned regulation of metabolic flux and redox balance.
The recombinant GOT1 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and enzymatic activity. Recombinant versions retain the native enzyme's ability to interconvert aspartate and α-ketoglutarate into oxaloacetate and glutamate, a reaction critical for maintaining cellular energy homeostasis and mitigating oxidative stress.
Research on GOT1 has expanded due to its implications in human diseases. Dysregulation of GOT1 is linked to cancer progression, particularly in tumors reliant on glutamine metabolism. It also plays a role in neurological disorders and liver diseases, where elevated GOT1 levels serve as clinical biomarkers. Recombinant GOT1 is widely used in *in vitro* studies to investigate metabolic pathways, screen enzyme inhibitors, or develop diagnostic assays.
Structurally, GOT1 functions as a homodimer, with each subunit binding pyridoxal 5'-phosphate (PLP) as a cofactor. Its recombinant form enables detailed biochemical studies, including crystallography and drug interaction analyses. By providing a pure, active enzyme source, recombinant GOT1 accelerates both basic research and therapeutic development, bridging gaps between metabolic biology and disease intervention strategies.
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