| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | RB; pRb; OSRC; pp110; p105-Rb |
| Entrez GeneID | 5925 |
| clone | 2A11D11 |
| WB Predicted band size | 106.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RB1 (AA: 745-928) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于RB1抗体的3篇参考文献示例(注：文献信息为模拟概括，实际引用请核对真实来源)：
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1. **文献名称**：*"Retinoblastoma protein partners in development and cancer"*
**作者**：Dyson, N.
**摘要**：该研究探讨了RB1蛋白在细胞周期调控中的相互作用网络，利用特异性RB1抗体进行免疫共沉淀(Co-IP)和Western blot分析，揭示了其在癌症中因突变导致的蛋白复合体功能失调。
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2. **文献名称**：*"Immunohistochemical analysis of RB1 expression in human tumors"*
**作者**：Friend, S.H. et al.
**摘要**：通过RB1抗体对多种肿瘤组织进行免疫组化(IHC)检测，发现RB1蛋白表达缺失与高级别恶性肿瘤(如小细胞肺癌和三阴性乳腺癌)显著相关，为预后评估提供了依据。
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3. **文献名称**：*"A monoclonal antibody specific for phosphorylated RB1 reveals cell cycle-dependent regulation"*
**作者**：Lee, W.H. & Bookstein, R.
**摘要**：开发了一种针对磷酸化RB1蛋白的单克隆抗体，用于流式细胞术和免疫荧光分析，证明RB1的磷酸化状态在G1/S期转换中起关键作用，并可用于化疗药物响应研究。
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4. **文献名称**：*"Validation of RB1 antibody specificity in CRISPR/Cas9-engineered knockout models"*
**作者**：Knudsen, E.S. et al.
**摘要**：利用CRISPR/Cas9构建RB1敲除细胞系，验证了多种商业化RB1抗体的特异性，为精准检测RB1蛋白表达及降解提供了标准化实验方案。
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提示：实际研究中建议通过PubMed或Google Scholar以关键词“RB1 antibody”、“retinoblastoma protein detection”检索最新文献。
The RB1 antibody is a crucial tool in studying the retinoblastoma protein (pRB), encoded by the RB1 gene, a pivotal tumor suppressor involved in cell cycle regulation. Discovered through research on retinoblastoma, a rare childhood eye cancer, RB1 mutations or deletions disrupt its function, leading to uncontrolled cell proliferation. The pRB protein primarily inhibits G1-S phase progression by binding and sequestering E2F transcription factors, thus blocking DNA replication. Its activity is modulated by phosphorylation, which releases E2F, enabling cell cycle progression. Dysfunctional RB1 is linked to various cancers, including osteosarcoma, lung, and bladder cancers.
RB1 antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and flow cytometry to detect pRB expression, localization, and phosphorylation status in tissues or cell lines. These antibodies aid in cancer research, diagnostics, and therapeutic development, helping assess tumor suppressor status or treatment responses. Specific clones target distinct epitopes or phosphorylation sites (e.g., Ser807/811), requiring careful selection based on experimental needs. Proper sample preparation is critical, as phosphorylation or degradation can affect detection. RB1 antibodies also contribute to studies on cell differentiation, apoptosis, and gene therapy strategies aiming to restore RB1 function in tumors. Their utility underscores RB1's central role in maintaining genomic stability and preventing oncogenesis.
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