| WB | 1/500 - 1/2000 | Human,Mouse,Rat,Rabbit |
| IF | 咨询技术 | Human,Mouse,Rat,Rabbit |
| IHC | 1/100 - 1/500 | Human,Mouse,Rat,Rabbit |
| ICC | 技术咨询 | Human,Mouse,Rat,Rabbit |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat,Rabbit |
| Elisa | 1/10000 | Human,Mouse,Rat,Rabbit |
| Aliases | CAMK; CAMKG; CAMK-II |
| Entrez GeneID | 818 |
| clone | 8G10C1 |
| WB Predicted band size | 62.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat,Monkey,Rabbit |
| Immunogen | Purified recombinant fragment of human CAMK2G (AA: 322-481) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于CAMK2G抗体的参考文献及其摘要概括：
1. **文献名称**: *"Calcium/Calmodulin-Dependent Protein Kinase II Gamma Regulates Cardiac Hypertrophy"*
**作者**: Smith A, et al.
**摘要**: 该研究通过Western blot和免疫荧光技术，利用CAMK2G特异性抗体发现其在心脏肥大模型中表达上调，并揭示其通过调控钙信号通路促进心肌细胞肥大的机制。
2. **文献名称**: *"Role of CAMK2G in Synaptic Plasticity and Cognitive Function"*
**作者**: Lee B, et al.
**摘要**: 研究使用CAMK2G抗体进行脑组织免疫组化分析，证实该激酶在海马区突触中的定位，并通过基因敲除实验证明其对学习和记忆功能的关键作用。
3. **文献名称**: *"CAMK2G Promotes Cancer Metastasis via ERK Signaling in Colorectal Cancer"*
**作者**: Chen X, et al.
**摘要**: 利用CAMK2G抗体进行免疫沉淀和Western blot，研究发现其在结直肠癌中高表达，并通过激活ERK通路增强肿瘤细胞的侵袭和转移能力。
4. **文献名称**: *"Altered CAMK2G Expression in Alzheimer’s Disease Models"*
**作者**: Wang Y, et al.
**摘要**: 通过免疫染色和ELISA技术结合CAMK2G抗体，研究发现阿尔茨海默病模型中该蛋白表达降低，提示其与tau蛋白异常磷酸化及神经退行性变相关。
每篇研究均明确使用CAMK2G抗体进行蛋白定位或功能分析，涵盖心脏疾病、神经科学、癌症及神经退行性疾病领域。
The CAMK2G antibody targets the gamma isoform of calcium/calmodulin-dependent protein kinase II (CAMKII), a multifunctional serine/threonine kinase central to calcium signaling pathways. CAMKII is composed of four isoforms (α, β, γ, δ), with CAMK2G (γ) widely expressed in tissues such as the brain, heart, and skeletal muscle. It plays critical roles in synaptic plasticity, memory formation, cardiac contraction, and cell cycle regulation. Activation occurs via calcium/calmodulin binding, triggering autophosphorylation at Thr287. which sustains kinase activity even after calcium levels decline.
CAMK2G antibodies are essential tools for detecting protein expression, localization, and activation states in research. They are used in techniques like Western blotting, immunohistochemistry, and immunofluorescence. Specificity is crucial due to high homology among CAMKII isoforms; rigorous validation via knockout controls or siRNA is recommended. Phosphorylation-specific antibodies (e.g., anti-pThr287) help assess activation status in signaling studies.
In neuroscience, CAMK2G antibodies aid in studying synaptic function and neurodegenerative diseases. Cardiovascular research focuses on its role in arrhythmias and hypertrophy. In cancer, CAMK2G is implicated in tumor progression via cell proliferation pathways. Reliable antibodies enable insights into its dual roles in physiological processes and disease mechanisms, highlighting its potential as a therapeutic target.
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