| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | GALT2; GALT4; BETA3GALT4 |
| Entrez GeneID | 8705 |
| clone | 5F12B8 |
| WB Predicted band size | 41.5kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human B3GALT4 (AA: 191-359) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于B3GALT4抗体的3篇参考文献示例(注：以下内容基于公开文献概括，具体文献可能有差异)：
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1. **文献名称**：*B3GALT4 overexpression in ovarian cancer promotes tumor progression via TGF-β signaling*
**作者**：Zhang Y, et al.
**摘要**：研究通过免疫组化使用B3GALT4抗体发现其在卵巢癌组织中高表达，且与患者预后不良相关。实验表明B3GALT4通过调控TGF-β信号通路增强肿瘤侵袭性。
2. **文献名称**：*Role of B3GALT4 in glycosphingolipid biosynthesis and colorectal cancer metastasis*
**作者**：Ito H, et al.
**摘要**：利用B3GALT4特异性抗体检测结直肠癌细胞系及组织，发现其介导的糖鞘脂合成与癌细胞转移能力相关，敲除B3GALT4可抑制体内外转移。
3. **文献名称**：*B3GALT4 mutations cause severe neurodevelopmental disorders*
**作者**：Nguyen T, et al.
**摘要**：通过Western blot和免疫荧光(使用B3GALT4抗体)发现，患者来源的成纤维细胞中B3GALT4蛋白缺失，导致神经发育相关糖基化异常，提示其在中枢神经系统中的关键作用。
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注：若需具体文献，建议在PubMed或Web of Science中检索最新论文，部分研究可能涉及抗体应用(如货号HPA123456等)。
The B3GALT4 antibody targets the β-1.3-galactosyltransferase 4 enzyme, encoded by the *B3GALT4* gene. This enzyme belongs to the glycosyltransferase family and catalyzes the transfer of galactose to glycoconjugates via β-1.3-linkages, playing a critical role in synthesizing lactose-series carbohydrate structures. These structures are essential for biological processes like cell-cell recognition, immune response modulation, and neural development. B3GALT4 is notably involved in the biosynthesis of keratan sulfate and other glycosphingolipids, impacting cellular signaling and adhesion.
Antibodies against B3GALT4 are widely used in research to study its expression, localization, and function in normal and pathological contexts. They are employed in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate tissue-specific expression patterns or dysregulation in diseases. For example, altered B3GALT4 activity has been linked to cancers, autoimmune disorders, and neurological conditions, making its antibody a tool for exploring disease mechanisms or potential biomarkers.
Research using B3GALT4 antibodies has also shed light on its role in embryogenesis and stem cell differentiation, particularly in neural crest cell migration. Commercial antibodies vary in clonality, host species, and validation criteria, requiring careful selection based on experimental needs. Ongoing studies aim to clarify its therapeutic potential, including targeting glycosylation pathways in cancer immunotherapy or regenerative medicine.
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