| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | PKR; PRKR; EIF2AK1 |
| Entrez GeneID | 5610 |
| clone | 3A10F7 |
| WB Predicted band size | 62kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human EIF2AK2 (AA: 329-551) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于EIF2AK2(PKR)抗体的3篇参考文献示例：
1. **"Role of PKR in the Antiviral Response"**
*作者：Williams, B.R. et al.*
**摘要**：研究了EIF2AK2(PKR)在病毒感染中的调控机制，通过Western blot和免疫荧光技术验证抗体特异性，发现PKR激活可抑制病毒复制。
2. **"PKR-Mediated Autophagy in Cancer Cells"**
*作者：Li, S. et al.*
**摘要**：利用EIF2AK2抗体进行免疫组化和流式细胞术，证明PKR通过调控自噬通路影响肿瘤细胞存活，为靶向治疗提供依据。
3. **"Phosphorylation Dynamics of EIF2AK2 in Stress Response"**
*作者：Garcia, M.A. et al.*
**摘要**：通过免疫沉淀和激酶活性实验，分析PKR在细胞应激中的磷酸化状态，其抗体成功用于检测内源性PKR的活化水平。
(注：以上文献为示例，实际引用需查询具体数据库获取真实研究。)
The EIF2AK2 antibody targets the protein kinase EIF2AK2. also known as PKR (Protein Kinase R), a critical regulator of innate immunity and cellular stress responses. EIF2AK2 is a double-stranded RNA (dsRNA)-dependent serine/threonine kinase activated during viral infections, inflammation, or oxidative stress. Upon binding viral or endogenous dsRNA, EIF2AK2 dimerizes and autophosphorylates, leading to its activation. This kinase phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (eIF2α), halting global protein synthesis to limit viral replication while selectively promoting translation of stress-response genes, such as ATF4.
EIF2AK2 antibodies are essential tools for studying its expression, activation, and interaction partners in pathways like NF-κB and apoptosis. Researchers use these antibodies in techniques like Western blot, immunofluorescence, and immunoprecipitation to explore EIF2AK2's roles in antiviral defense, tumor suppression, and neurodegenerative diseases. Dysregulation of EIF2AK2 has been linked to cancer, chronic inflammation, and Alzheimer’s disease, making its antibody valuable for diagnostic and therapeutic research. Commercial EIF2AK2 antibodies are typically validated for specificity across human, mouse, and rat samples, with some targeting phosphorylated forms (e.g., Thr451/Thr446) to assess activation status. Understanding EIF2AK2's dual role in cell survival and death continues to drive interest in targeting this kinase for drug development.
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