| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Entrez GeneID | 5617 |
| clone | 2H10B2 |
| WB Predicted band size | 26kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human PRL (AA: 65-173) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于PRL(催乳素)抗体的模拟参考文献示例(注：以下内容为虚构，仅用于格式参考，建议通过学术数据库获取真实文献)：
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1. **文献名称**：*Development of a High-Specificity Monoclonal Antibody for Human Prolactin Detection*
**作者**：Smith, J. et al.
**摘要**：本研究开发了一种针对人催乳素(PRL)的单克隆抗体，通过杂交瘤技术筛选出高亲和力抗体(KD=0.8 nM)，并验证其在血清ELISA检测中的特异性，显著降低与生长激素(GH)的交叉反应，适用于临床高催乳素血症诊断。
2. **文献名称**：*Prolactin Autoantibodies in Infertility: A Clinical and Molecular Analysis*
**作者**：Johnson, R. et al.
**摘要**：通过免疫印迹法分析不明原因不孕症患者的血清，发现15%样本存在PRL自身抗体，这些抗体可能干扰PRL的生物活性，导致内分泌紊乱，提示其在生殖障碍中的潜在病理作用。
3. **文献名称**：*A Novel Polyclonal Antibody for Prolactin Receptor Co-localization Studies in Breast Cancer*
**作者**：Lee, H. et al.
**摘要**：利用重组人PRL受体胞外域制备兔多克隆抗体，通过免疫组化验证其在乳腺癌组织中的定位能力，证实PRL受体与EGFR共表达，为靶向治疗提供新工具。
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**建议**：实际文献可通过PubMed、Google Scholar等平台，以关键词“prolactin antibody”、“PRL immunoassay”或“prolactin autoantibody”检索。
Prolactin (PRL) antibodies are immunological tools designed to detect and quantify PRL, a peptide hormone primarily secreted by the anterior pituitary gland. PRL plays critical roles in lactation, reproduction, metabolism, and immune regulation. Its dysregulation is linked to conditions like hyperprolactinemia, pituitary adenomas, and reproductive disorders. PRL antibodies are essential in both research and clinical diagnostics. In research, they enable the study of PRL expression, localization, and function via techniques such as ELISA, Western blotting, and immunohistochemistry. Clinically, they aid in diagnosing PRL-secreting tumors and monitoring treatment responses.
These antibodies are typically generated by immunizing animals (e.g., rabbits, mice) with purified PRL protein or synthetic PRL-derived peptides, followed by hybridoma technology for monoclonal antibody production. Specificity and affinity are validated through cross-reactivity tests with related hormones (e.g., growth hormone, placental lactogen) to ensure minimal interference. Recent advancements include recombinant PRL antibodies engineered for enhanced consistency and reduced batch variability. Challenges include distinguishing between monomeric PRL (23 kDa) and its isoforms (e.g., macroprolactin), which can affect assay accuracy. PRL antibodies also facilitate exploration of extrapituitary PRL roles in tissues like the mammary gland, brain, and immune cells. Their continued refinement supports precision in endocrine research and improves diagnostic reliability for PRL-associated pathologies.
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