| WB | 1/500 - 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | HPRT; HGPRT |
| Entrez GeneID | 3251 |
| clone | 5F11A7 |
| WB Predicted band size | 24.6kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human HPRT1 (AA: FULL(1-218)) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于HPRT1抗体的3篇参考文献及其简要摘要：
---
1. **"Hypoxanthine-guanine phosphoribosyltransferase (HPRT) deficiency: Lesch-Nyhan syndrome"**
*作者：Jinnah, H.A., Friedmann, T.*
**摘要**：该综述探讨了HPRT1基因突变导致的Lesch-Nyhan综合征的分子机制，强调了HPRT1抗体在检测患者细胞中酶活性缺失及蛋白表达异常中的应用，为疾病诊断提供依据。
---
2. **"Tissue-specific expression and cDNA cloning of human HPRT"**
*作者：Edwards, A., Voss, H., Rice, P., et al.*
**摘要**：研究通过HPRT1抗体进行免疫印迹和免疫组化分析，揭示了HPRT1蛋白在不同人类组织中的表达差异，并成功克隆了其cDNA，为后续功能研究奠定基础。
---
3. **"Housekeeping proteins: Validation of reference genes for Western blotting in human cells"**
*作者：Almén, M.S., Bringeland, N., Fredriksson, R.*
**摘要**：评估HPRT1作为Western Blot内参的稳定性，证明其抗体在不同实验条件下(如药物处理)的可靠性，优于传统内参(如β-actin)，适用于基因表达标准化分析。
---
这些文献涵盖了HPRT1抗体在疾病机制研究、蛋白表达分析和实验方法标准化中的应用。如需更多文献或具体细节，可进一步检索PubMed或SciFinder数据库。
The hypoxanthine phosphoribosyltransferase 1 (HPRT1) antibody is a critical tool for studying the HPRT1 enzyme, encoded by the *HPRT1* gene. This enzyme plays a central role in the purine salvage pathway, catalyzing the conversion of hypoxanthine and guanine into their respective nucleoside monophosphates (IMP and GMP), which are essential for DNA/RNA synthesis and cellular energy metabolism. HPRT1 is widely used as a housekeeping protein in molecular biology due to its consistent expression across tissues, making it a common loading control in Western blotting and normalization reference in qPCR.
HPRT1 deficiency is linked to severe genetic disorders like Lesch-Nyhan syndrome, characterized by neurological dysfunction and hyperuricemia, as well as gouty arthritis. Researchers employ HPRT1 antibodies to investigate these conditions, monitor protein expression in disease models, or validate gene-editing outcomes (e.g., CRISPR knockouts). These antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes of human HPRT1. and are validated for applications including immunohistochemistry, flow cytometry, and ELISA. Cross-reactivity with homologs in common model organisms (e.g., mouse, rat) is often confirmed to support translational research. Quality validation via knockdown/knockout samples ensures specificity, critical for accurate data interpretation.
×
