| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/200 - 1/1000 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | TRIM63; IRF; SMRZ; MURF2; RNF28 |
| Entrez GeneID | 84676 |
| clone | 6H6D4 |
| WB Predicted band size | 40.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human MuRF1 (AA: 293-304). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于MuRF1抗体的经典文献概览，聚焦其功能研究与实验应用：
1. **"Atrogin-1/MuRF1 regulates ubiquitin ligase activity through multiple mechanisms"**
*作者：Bodine SC et al. (2006)*
摘要：验证MuRF1抗体在肌肉萎缩模型(如去神经化)中的特异性，揭示其通过泛素化降解肌球蛋白重链的分子机制。
2. **"The E3 ligase MuRF1 protects against cardiac ischemia-reperfusion injury through stabilization of ATP synthase"**
*作者：Zhang G et al. (2018)*
摘要：利用MuRF1敲除小鼠及特异性抗体，发现MuRF1通过调控线粒体ATP酶保护心肌细胞，抗体应用于免疫共沉淀验证靶蛋白互作。
3. **"MuRF1-mediated proteolysis in skeletal muscle atrophy"**
*作者：Clarke BA et al. (2007)*
摘要：通过Western blot和免疫荧光技术，证实MuRF1抗体在不同萎缩模型中(如糖皮质激素诱导)的敏感性，揭示其转录后调控机制。
注：建议通过PubMed或期刊官网输入标题获取完整文献。如需实验抗体品牌推荐，可补充说明具体应用场景(如物种、实验类型)。
The Muscle RING-finger protein-1 (MuRF1), encoded by the *TRIM63* gene, is a muscle-specific E3 ubiquitin ligase that plays a critical role in protein ubiquitination and degradation via the ubiquitin-proteasome system (UPS). Primarily expressed in skeletal and cardiac muscles, MuRF1 is a key regulator of muscle atrophy under conditions such as denervation, fasting, cancer cachexia, and sepsis. It targets structural proteins like titin, myosin heavy chains, and troponin I for degradation, leading to muscle wasting. Research on MuRF1 has surged due to its involvement in pathological and physiological muscle remodeling, making it a biomarker for muscle atrophy and a therapeutic target for diseases like muscular dystrophy, heart failure, and age-related sarcopenia.
MuRF1 antibodies are essential tools for detecting and quantifying MuRF1 expression in research. These antibodies, often developed in rabbits or mice, are validated for applications including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and immunoprecipitation (IP). Specificity and sensitivity vary among commercial antibodies, requiring careful validation using knockout controls. MuRF1 antibodies have enabled discoveries in molecular pathways linking inflammation, oxidative stress, and hormonal signaling to muscle degradation. Commercially available from suppliers like Cell Signaling Technology, Abcam, and Santa Cruz Biotechnology, these antibodies continue to support studies aiming to modulate MuRF1 activity to counteract muscle wasting in clinical settings.
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