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Test parameter | specification | test result | ||||||
Positive control | >0.8 | 1.256 | ||||||
Negative control | <0.2 | 0.163 | ||||||
Positive rate | 20,Positive | 100% | ||||||
Negative rate | 20,Negative | 100% |
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A 96-well Coated assay plate 1 -- This microplate has been pre-coated with human SARS-CoV-2 S1 RBD antigen.
Negative Control (1 x 800 μl) -- It is free of the SARS-CoV-2 S1 RBD IgG antibody and used to preclude the false positive.
Positive Control (1 x 800 μl) -- Used to evaluate the validity, stability, and comparability of the test results.
HRP-conjugated anti-Human IgG antibody (100 x concentrate) (1 x 120 μl) -- Act as the detection antibody.
HRP-conjugate Diluent (1 x 20 ml) -- Dilute the HRPconjugated anti-Human IgG antibody.
Sample Diluent (2 x 20 ml) -- Dilute the sample solution.
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer.
Absorbent paper for blotting the microtiter plate.
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