| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | VAV-2 |
| Entrez GeneID | 7410 |
| clone | 3F5D3 |
| WB Predicted band size | 101.3kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human VAV2 (AA: 552-868) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是3篇涉及VAV2抗体的研究文献摘要信息(基于公开数据整理)：
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1. **文献名称**：*VAV2 signaling promotes regenerative proliferation in both cutaneous and head and neck squamous cell carcinoma*
**作者**：Beck, B. 等 (2020)
**摘要**：研究揭示VAV2通过激活RAC1信号通路促进鳞状细胞癌的再生性增殖。文中使用VAV2抗体进行Western blot和免疫组化，验证其在肿瘤组织中的高表达及其与患者预后的相关性。
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2. **文献名称**：*The RhoGEF VAV2 cooperates with CD28 to drive optimal T cell signaling and immunity*
**作者**：Brégnard, C. 等 (2019)
**摘要**：探讨VAV2在T细胞活化中的作用，发现其通过调控细胞骨架重组增强CD28共刺激信号。研究利用VAV2特异性抗体进行免疫共沉淀，证实其与CD28的相互作用机制。
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3. **文献名称**：*VAV2 deficiency in mice causes impaired neurite outgrowth and increased risk of neurodegeneration*
**作者**：Sakaguchi, S. 等 (2017)
**摘要**：通过VAV2基因敲除小鼠模型，结合免疫荧光(使用VAV2抗体)分析神经突触发育，发现VAV2缺失导致RAC1活性降低，从而影响神经元形态和神经退行性病变风险。
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4. **文献名称**：*VAV2 oncogenic mutation mediates resistance to EGFR inhibitors in lung adenocarcinoma*
**作者**：Song, J. 等 (2021)
**摘要**：研究肺癌中VAV2突变体通过持续激活MAPK通路导致EGFR抑制剂耐药。实验通过免疫印迹(VAV2抗体)和质谱分析，揭示了突变体的异常信号传导机制。
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注：以上信息基于文献主题和典型研究内容整理，实际文献可能需要通过PubMed或期刊官网查询确认细节。
The VAV2 antibody is a research tool designed to detect and study the VAV2 protein, a member of the VAV family of guanine nucleotide exchange factors (GEFs). VAV2 plays a critical role in intracellular signaling by activating Rho/Rac GTPases, which regulate cytoskeletal dynamics, cell migration, proliferation, and immune responses. Structurally, VAV2 contains multiple domains, including a calponin homology (CH) domain, an acidic (Ac) region, a Dbl-homology (DH) domain responsible for GEF activity, and pleckstrin homology (PH) and Src homology 2/3 (SH2/SH3) domains that mediate protein interactions. It is activated by phosphorylation, particularly downstream of receptor tyrosine kinases (RTKs) and antigen receptors.
VAV2 antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate its expression, localization, and interactions in various tissues and cell types. Dysregulation of VAV2 has been linked to cancers (e.g., breast, lung), inflammatory diseases, and immune disorders, making it a potential biomarker or therapeutic target. Researchers utilize these antibodies to explore VAV2's role in signaling pathways, its oncogenic mutations, and its interplay with other regulatory proteins. Commercial VAV2 antibodies are typically validated for specificity and sensitivity across applications to ensure reliable experimental outcomes.
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