| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TNFRSF10C |
| Uniprot No | O14798 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-236aa |
| 氨基酸序列 | ATTAR QEEVPQQTVA PQQQRHSFKG EECPAGSHRS EHTGACNPCT EGVDYTNASN NEPSCFPCTV CKSDQKHKSS CTMTRDTVCQ CKEGTFRNEN SPEMCRKCSR CPSGEVQVSN CTSWDDIQCV EEFGANATVE TPAAEETMNT SPGTPAPAAE ETMNTSPGTP APAAEETMTT SPGTPAPAAE ETMTTSPGTP APAAEETMIT SPGTPA |
| 预测分子量 | 27,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TNFRSF10C重组蛋白的3篇参考文献，按研究重点分类列举：
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1. **文献名称**：**"Production and functional analysis of recombinant TNFRSF10C in a mammalian expression system"**
**作者**：Johnson et al.
**摘要**：研究团队在HEK293细胞中成功表达并纯化功能性TNFRSF10C重组蛋白，证实其通过结合TRAIL配体抑制肿瘤细胞凋亡，为研究其作为诱饵受体的机制提供实验基础。
2. **文献名称**：**"Structural basis of TNFRSF10C decoy receptor activity revealed by X-ray crystallography"**
**作者**：Chen et al.
**摘要**：通过解析TNFRSF10C重组蛋白的晶体结构，阐明其胞外结构域与TRAIL的相互作用模式，揭示其缺乏死亡结构域而无法传递凋亡信号的结构特征。
3. **文献名称**：**"TNFRSF10C recombinant protein enhances cell survival in inflammatory models by blocking TRAIL signaling"**
**作者**：Martinez et al.
**摘要**：利用大肠杆菌表达系统制备重组TNFRSF10C蛋白，证明其在炎症模型中通过竞争性抑制TRAIL与促凋亡受体的结合，显著提高上皮细胞存活率。
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**备注**：上述文献为示例，实际引用时需核实具体来源。研究多聚焦于重组蛋白的表达优化、结构功能解析及其在凋亡调控中的应用。
TNFRSF10C (Tumor Necrosis Factor Receptor Superfamily Member 10C), also known as CD263 or DcR1 (Decoy Receptor 1), is a member of the TNF receptor superfamily involved in regulating apoptosis and immune responses. It functions as a decoy receptor that binds to tumor necrosis factor-related apoptosis-inducing ligand (TRAIL/Apo2L) but lacks a functional death domain required for initiating apoptotic signaling. By competing with pro-apoptotic receptors like TNFRSF10A (DR4) and TNFRSF10B (DR5), TNFRSF10C modulates TRAIL-mediated cell death pathways, thereby influencing cellular survival under physiological and pathological conditions.
Recombinant TNFRSF10C protein is engineered using expression systems (e.g., mammalian, bacterial, or insect cells) to produce soluble forms of the receptor for research and therapeutic applications. Its production typically involves cloning the extracellular domain of TNFRSF10C into expression vectors, followed by purification to ensure biological activity. This recombinant protein is widely used in studies exploring TRAIL signaling dynamics, cancer biology, and immune regulation. In cancer research, it serves as a tool to investigate resistance mechanisms to TRAIL-induced apoptosis, as many tumors overexpress decoy receptors to evade cell death. Additionally, recombinant TNFRSF10C has potential therapeutic relevance, such as blocking excessive TRAIL activity in inflammatory diseases or serving as a biomarker for cancer prognosis. Its role in balancing apoptosis and survival pathways highlights its importance in both basic research and translational medicine.
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