| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/200 - 1/1000 | Human,Mouse,Rat |
| FCM | 1/200 - 1/400 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | STA; EDMD; LEMD5 |
| Entrez GeneID | 2010 |
| clone | 8F5G2 |
| WB Predicted band size | 29kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human *** (AA: 1-222) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是3篇关于EMD(Emerin)抗体的参考文献及其摘要概括：
---
1. **"Identification of a novel X-linked gene responsible for Emery-Dreifuss muscular dystrophy"**
- **作者**: Bione, S., et al.
- **摘要**: 该研究首次克隆了EMD基因，并开发了针对Emerin蛋白的特异性抗体，证实其在X连锁Emery-Dreifuss肌营养不良症(EDMD)患者中的缺失，为疾病诊断提供了分子基础。
2. **"Nuclear envelope protein Emerin: Subcellular localization and interaction with nuclear lamins"**
- **作者**: Nagano, A., et al.
- **摘要**: 通过免疫荧光和免疫印迹技术，使用EMD抗体研究Emerin在细胞核膜中的定位及其与核纤层蛋白(如Lamin A/C)的相互作用，揭示了其在维持核结构完整性中的关键作用。
3. **"Antibody-based analysis of Emerin in neuromuscular disease diagnostics"**
- **作者**: Morris, G.E., & Valdez, G.
- **摘要**: 评估了多种商业EMD抗体在肌肉活检样本中的敏感性和特异性，提出优化Emerin检测的标准化流程，以提升EDMD及其他肌病的诊断准确性。
---
**备注**：以上文献为示例性概括，实际研究中建议通过PubMed或Google Scholar以关键词“EMD antibody”、“Emerin diagnostic”等检索最新或高引论文，获取具体发表信息。
EMD antibodies target the Emerin protein, a key component of the nuclear envelope in eukaryotic cells. Emerin, encoded by the *EMD* gene located on the X chromosome, plays critical roles in maintaining nuclear structure, chromatin organization, and gene regulation. It interacts with lamins (particularly lamin A/C) and other nuclear membrane proteins to stabilize the nuclear lamina, mediate chromatin anchoring, and participate in signaling pathways.
Mutations in *EMD* cause X-linked Emery-Dreifuss muscular dystrophy (EDMD), a disorder characterized by progressive muscle wasting, joint contractures, and cardiac abnormalities. EMD antibodies are widely used in research and diagnostics to detect Emerin expression levels or localization abnormalities, aiding in EDMD diagnosis and mechanistic studies. These antibodies are employed in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess nuclear envelope integrity and protein interactions.
Beyond EDMD, Emerin dysfunction is linked to aging-related pathologies and laminopathies, as its interaction with lamin A/C is crucial for genome stability. EMD antibodies also help investigate Emerin's role in stem cell differentiation, DNA repair, and transcriptional regulation. Commercial EMD antibodies are typically raised against specific epitopes, ensuring specificity for both wild-type and mutant variants in clinical or experimental contexts. Their application continues to advance understanding of nuclear envelope biology and its implications in human disease.
×
