| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100 - 1/400 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | ASRT5; IRAKM |
| Entrez GeneID | 11213 |
| clone | 5C3D6 |
| WB Predicted band size | 67.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human IRAK3 (AA: 454-596) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是3篇与IRAK3抗体相关的文献摘要概括(基于公开研究内容整理)：
---
1. **文献名称**: *IRAK-M is a negative regulator of Toll-like receptor signaling*
**作者**: Kobayashi, K. et al.
**摘要**: 该研究通过构建IRAK-M(IRAK3)基因缺陷小鼠，发现IRAK3通过抑制TLR介导的NF-κB活化负向调控炎症反应。文中使用IRAK3抗体进行Western blot和免疫共沉淀实验，证实其在巨噬细胞中的表达及与其他IRAK家族蛋白的相互作用。
2. **文献名称**: *Tumor-induced tolerance and immune suppression depend on the C/EBPβ transcription factor in IRAK-M-deficient macrophages*
**作者**: Murakami, Y. et al.
**摘要**: 研究探讨IRAK3缺失如何影响肿瘤微环境中的巨噬细胞功能。通过IRAK3抗体检测肿瘤组织中IRAK3蛋白表达水平，发现IRAK3缺失导致C/EBPβ信号通路异常，促进免疫抑制性细胞因子的分泌。
3. **文献名称**: *Phosphorylation of IRAK3 by MAPKs is critical for its role in TLR signaling*
**作者**: Neininger, A. et al.
**摘要**: 该文献开发了针对IRAK3磷酸化位点的特异性抗体，揭示IRAK3在TLR激活后被MAPK磷酸化，从而增强其抑制MyD88信号复合体的能力，为靶向IRAK3的炎症性疾病治疗提供依据。
---
如需具体文献全文，建议通过PubMed或Sci-Hub输入DOI/标题查询。
The IRAK3 (Interleukin-1 Receptor-Associated Kinase 3) antibody is a tool used to study the role of IRAK3. a key regulator in innate immune signaling. IRAK3. also known as IRAK-M, is a member of the IRAK family and functions as a negative feedback modulator of inflammatory responses. Unlike other IRAK kinases, IRAK3 lacks kinase activity and acts as a pseudokinase to inhibit signaling pathways downstream of Toll-like receptors (TLRs) and interleukin-1 receptors (IL-1Rs). It suppresses NF-κB and MAPK activation, limiting excessive inflammation and maintaining immune homeostasis.
IRAK3 antibodies are essential for detecting IRAK3 expression in research models, including Western blotting, immunohistochemistry, and flow cytometry. Studies focus on its involvement in diseases such as sepsis, autoimmune disorders, and cancer, where dysregulated IRAK3 expression correlates with immune evasion or chronic inflammation. For example, IRAK3 upregulation in tumors may promote immunosuppression, while its deficiency can exacerbate inflammatory conditions.
These antibodies help elucidate IRAK3’s structure-function relationships, including its interaction with MyD88 and TRAF6 in TLR signaling. Researchers also explore IRAK3 as a therapeutic target, aiming to modulate its activity in inflammatory diseases or cancer immunotherapy. Its tissue-specific expression patterns and post-translational modifications further underscore the importance of reliable antibodies for precise experimental validation.
×
