| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GSTM4 |
| Uniprot No | Q03013 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-218aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSMTLGYWDIRGLAHAIRLLLEYTDSSYEE KKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYI ARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKPEYLEE LPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPN LKDFISRFEGLEKISAYMKSSRFLPKPLYTRVAVWGNK |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GSTM4重组蛋白的3篇参考文献及其摘要内容：
1. **文献名称**：*"Expression and purification of recombinant human GSTM4 and its enzymatic characterization"*
**作者**：Zhang Y, et al.
**摘要**：该研究通过大肠杆菌表达系统成功纯化了重组人源GSTM4蛋白，并分析了其对不同底物的谷胱甘肽转移酶活性，揭示了其在代谢解毒中的潜在功能。
2. **文献名称**：*"Overexpression of GSTM4 in hepatocellular carcinoma and its role in chemoresistance"*
**作者**：Lee S, Kim DH.
**摘要**：研究发现肝癌细胞中GSTM4重组蛋白的过表达与化疗药物(如顺铂)耐药性相关，通过调控氧化应激通路增强癌细胞存活能力。
3. **文献名称**：*"Structural insights into the substrate specificity of GSTM4 through crystallographic analysis"*
**作者**：Wang X, et al.
**摘要**：通过X射线晶体学解析了重组GSTM4蛋白的三维结构，明确了其底物结合口袋的关键氨基酸残基，为设计靶向抑制剂提供了结构基础。
4. **文献名称**：*"GSTM4 modulates oxidative stress in neurodegenerative models: Implications for Parkinson’s disease"*
**作者**：Brown CM, et al.
**摘要**：利用重组GSTM4蛋白研究其在神经元氧化应激中的保护作用，发现其通过减少脂质过氧化物积累缓解帕金森病模型中的细胞损伤。
(注：以上文献为示例，实际引用需根据具体研究检索PubMed或Web of Science获取。)
**Background of GSTM4 Recombinant Protein**
Glutathione S-transferase Mu 4 (GSTM4) is a member of the glutathione S-transferase (GST) superfamily, a group of phase II detoxification enzymes that catalyze the conjugation of glutathione (GSH) to electrophilic substrates. This process neutralizes reactive oxygen species (ROS), xenobiotics, and carcinogens, playing a critical role in cellular protection against oxidative stress and chemical toxicity. The GSTM subfamily, including GSTM4. is primarily expressed in tissues such as the liver, kidneys, and lungs, where detoxification processes are highly active.
GSTM4 recombinant protein is produced through genetic engineering techniques, typically by inserting the GSTM4 gene into expression vectors (e.g., bacterial or mammalian systems) to enable large-scale production. This engineered protein retains the functional properties of native GSTM4. including substrate-binding specificity and enzymatic activity. Its recombinant form allows researchers to study GSTM4’s structure-function relationships, catalytic mechanisms, and interactions with inhibitors or therapeutic compounds in controlled experimental settings.
Research on GSTM4 has gained attention due to its potential role in cancer biology. Overexpression or polymorphisms in GSTM4 have been linked to altered drug metabolism and chemoresistance in certain cancers, suggesting its involvement in tumor progression and treatment response. Additionally, GSTM4’s ability to modulate oxidative stress pathways highlights its relevance in neurodegenerative diseases and aging-related disorders.
The availability of GSTM4 recombinant protein facilitates drug discovery, toxicology studies, and biomarker development. It is widely used in enzymatic assays, protein interaction studies, and as a standard in quantitative analyses. Despite its established roles, further investigations are needed to fully elucidate its tissue-specific functions and therapeutic targeting potential. Overall, GSTM4 recombinant protein serves as a vital tool for advancing understanding of detoxification biology and disease mechanisms.
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