| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCF7 |
| Uniprot No | P36402 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-384aa |
| 氨基酸序列 | MPQLDSGGGGAGGGDDLGAPDELLAFQDEGEEQDDKSRDSAAGPERDLAELKSSLVNESEGAAGGAGIPGVPGAGAGARGEAEALGREHAAQRLFPDKLPEPLEDGLKAPECTSGMYKETVYSAFNLLMHYPPPSGAGQHPQPQPPLHKANQPPHGVPQLSLYEHFNSPHPTPAPADISQKQVHRPLQTPDLSGFYSLTSGSMGQLPHTVSWFTHPSLMLGSGVPGHPAAIPHPAIVPPSGKQELQPFDRNLKTQAESKAEKEAKKPTIKKPLNAFMLYMKEMRAKVIAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKRRSREKHQESTTETNWPRELKDGNGQESLSMSSSSSPA |
| 预测分子量 | 49.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCF7重组蛋白的3篇参考文献示例(注：部分信息为示例性概括，实际文献可能存在差异)：
1. **文献名称**：*TCF7重组蛋白的克隆、表达及与β-catenin的相互作用研究*
**作者**：Smith A, et al.
**摘要**：该研究通过在大肠杆菌中表达并纯化重组TCF7蛋白，利用Pull-down实验和荧光共振能量转移(FRET)技术验证了TCF7与β-catenin的直接结合，为Wnt信号通路的分子机制提供了实验依据。
2. **文献名称**：*Structural analysis of TCF7 reveals DNA-binding motifs critical for transcriptional regulation*
**作者**：Lee J, et al.
**摘要**：作者通过重组TCF7蛋白的结晶和X射线衍射分析，解析了其三维结构，发现其HMG结构域的关键氨基酸残基对DNA结合活性至关重要，并进一步通过突变实验验证了功能影响。
3. **文献名称**：*TCF7重组蛋白在T淋巴细胞分化中的功能验证*
**作者**：Wang X, et al.
**摘要**：研究利用体外表达的TCF7重组蛋白，结合报告基因实验和染色质免疫沉淀(ChIP)，证明TCF7通过调控特定靶基因(如CD4/CD8)的表达参与T细胞分化过程。
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**注**：以上文献为示例性内容，实际引用时请根据具体研究通过学术数据库(如PubMed、Web of Science)检索并核实原文信息。
**Background of TCF7 Recombinant Protein**
TCF7 (T-cell factor 7), also known as TCF1. is a member of the T-cell factor/lymphoid enhancer factor (TCF/LEF) family of transcription factors. These proteins play pivotal roles in the Wnt/β-catenin signaling pathway, a conserved mechanism critical for embryonic development, cell proliferation, stem cell maintenance, and tissue homeostasis. TCF7 functions as a nuclear mediator of Wnt signals by binding to β-catenin, which translocates to the nucleus upon pathway activation. Together, the TCF7/β-catenin complex regulates the expression of Wnt target genes, such as *c-Myc* and *Cyclin D1*, driving processes like cell cycle progression and differentiation.
Dysregulation of TCF7 is implicated in various pathologies, particularly cancers. Overactivation of Wnt signaling, often due to mutations in pathway components (e.g., APC, β-catenin), leads to uncontrolled cell growth and is linked to colorectal cancer, leukemia, and other malignancies. TCF7 also influences immune cell development, notably in T-cell differentiation and thymocyte maturation, highlighting its diverse functional roles.
Recombinant TCF7 protein is engineered for research applications, typically produced in *E. coli* or mammalian expression systems to ensure proper folding and post-translational modifications. It is often purified via affinity tags (e.g., His-tag) and validated for activity in DNA-binding assays or Wnt-responsive reporter systems. Researchers utilize recombinant TCF7 to study Wnt pathway dynamics, protein-protein/DNA interactions, and mechanisms of oncogenesis. Additionally, it serves as a tool for screening inhibitors targeting the TCF/β-catenin interaction, a therapeutic strategy for Wnt-driven cancers.
Overall, TCF7 recombinant protein is a vital reagent for dissecting Wnt signaling biology and developing targeted therapies against diseases associated with pathway dysregulation.
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